4EB8
Crystal structure of purine nucleoside phosphorylase (W16Y, W94Y, W178Y, H257W) mutant from human complexed with DADMe-ImmG and phosphate
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C (A, B, C) | Purine nucleoside phosphorylase | polymer | 324 | 36127.1 | 3 | UniProt (P00491) Pfam (PF01048) | Homo sapiens (human) | PNP, Inosine phosphorylase |
| 2 | D, F, I (A, B, C) | PHOSPHATE ION | non-polymer | 95.0 | 3 | Chemie (PO4) | |||
| 3 | E, G, J (A, B, C) | 2-amino-7-{[(3R,4R)-3-hydroxy-4-(hydroxymethyl)pyrrolidin-1-yl]methyl}-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one | non-polymer | 279.3 | 3 | Chemie (IM5) | |||
| 4 | H, K (B, C) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 2 | Chemie (EDO) | |||
| 5 | L, M, N (A, B, C) | water | water | 18.0 | 323 | Chemie (HOH) |
Sequence modifications
A, B, C: 1 - 289 (UniProt: P00491)
| PDB | External Database | Details |
|---|---|---|
| Met -34 | - | initiating methionine |
| Arg -33 | - | expression tag |
| Gly -32 | - | expression tag |
| Ser -31 | - | expression tag |
| His -30 | - | expression tag |
| His -29 | - | expression tag |
| His -28 | - | expression tag |
| His -27 | - | expression tag |
| His -26 | - | expression tag |
| His -25 | - | expression tag |
| Gly -24 | - | expression tag |
| Met -23 | - | expression tag |
| Ala -22 | - | expression tag |
| Ser -21 | - | expression tag |
| Met -20 | - | expression tag |
| Thr -19 | - | expression tag |
| Gly -18 | - | expression tag |
| Gly -17 | - | expression tag |
| Gln -16 | - | expression tag |
| Gln -15 | - | expression tag |
| Met -14 | - | expression tag |
| Gly -13 | - | expression tag |
| Arg -12 | - | expression tag |
| Asp -11 | - | expression tag |
| Leu -10 | - | expression tag |
| Tyr -9 | - | expression tag |
| Asp -8 | - | expression tag |
| Asp -7 | - | expression tag |
| Asp -6 | - | expression tag |
| Asp -5 | - | expression tag |
| Lys -4 | - | expression tag |
| Asp -3 | - | expression tag |
| Pro -2 | - | expression tag |
| Thr -1 | - | expression tag |
| Leu 0 | - | expression tag |
| Tyr 16 | Trp 16 | engineered mutation |
| Ser 51 | Gly 51 | engineered mutation |
| Tyr 94 | Trp 94 | engineered mutation |
| Tyr 178 | Trp 178 | engineered mutation |
| Trp 257 | His 257 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 108381.4 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 1246.9 | |
| All* | Total formula weight | 109628.3 |
