Crystal structure of purine nucleoside phosphorylase (W16Y, W94Y, W178Y, H257W) mutant from human complexed with DADMe-ImmG and phosphate

Summary for 4EAR

DescriptorPurine nucleoside phosphorylase, 2-amino-7-{[(3R,4R)-3-hydroxy-4-(hydroxymethyl)pyrrolidin-1-yl]methyl}-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one, PHOSPHATE ION, ... (4 entities in total)
Functional Keywordspnp, immucillin, purine nucleoside phosphorylase, nucleoside binding, purine base binding, purine-nucleoside phosphorylase activity, drug binding, transferase activity, transferring glycosyl groups, phosphate ion binding, cytosol, 6-fluoro-l-tryptophan, transferase-transferase inhibitor complex, transferase/transferase inhibitor
Biological sourceHomo sapiens (human)
Cellular locationCytoplasm, cytoskeleton (By similarity) P00491
Total number of polymer chains3
Total molecular weight109558.15
Haapalainen, A.M.,Ho, M.C.,Suarez, J.J.,Almo, S.C.,Schramm, V.L. (deposition date: 2012-03-22, release date: 2013-02-06, Last modification date: 2013-03-13)
Primary citation
Suarez, J.,Haapalainen, A.M.,Cahill, S.M.,Ho, M.C.,Yan, F.,Almo, S.C.,Schramm, V.L.
Catalytic Site Conformations in Human PNP by (19)F-NMR and Crystallography.
Chem.Biol., 20:212-222, 2013
PubMed: 23438750 (PDB entries with the same primary citation)
DOI: 10.1016/j.chembiol.2013.01.009
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.210200.7%10.7%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
Download full validation reportDownload
PDB entries from 2020-07-29