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4DJZ

Catalytic fragment of masp-1 in complex with its specific inhibitor developed by directed evolution on sgci scaffold

4DJZ の概要
エントリーDOI10.2210/pdb4djz/pdb
関連するPDBエントリー3TVJ
分子名称Mannan-binding lectin serine protease 1 heavy chain, Mannan-binding lectin serine protease 1 light chain, Protease inhibitor SGPI-2, ... (4 entities in total)
機能のキーワードin vitro evolution, specific inhibitor, hydrolase, hydrolase-hydrolase inhibitor complex, hydrolase/hydrolase inhibitor
由来する生物種Homo sapiens (human)
詳細
細胞内の位置Secreted: P48740 P48740 O46162
タンパク質・核酸の鎖数6
化学式量合計99251.53
構造登録者
Heja, D.,Harmat, V.,Fodor, K.,Wilmanns, M.,Dobo, J.,Kekesi, K.A.,Zavodszky, P.,Gal, P.,Pal, G. (登録日: 2012-02-03, 公開日: 2012-04-25, 最終更新日: 2024-11-06)
主引用文献Heja, D.,Harmat, V.,Fodor, K.,Wilmanns, M.,Dobo, J.,Kekesi, K.A.,Zavodszky, P.,Gal, P.,Pal, G.
Monospecific Inhibitors Show That Both Mannan-binding Lectin-associated Serine Protease-1 (MASP-1) and -2 Are Essential for Lectin Pathway Activation and Reveal Structural Plasticity of MASP-2.
J.Biol.Chem., 287:20290-20300, 2012
Cited by
PubMed Abstract: The lectin pathway is an antibody-independent activation route of the complement system. It provides immediate defense against pathogens and altered self-cells, but it also causes severe tissue damage after stroke, heart attack, and other ischemia reperfusion injuries. The pathway is triggered by target binding of pattern recognition molecules leading to the activation of zymogen mannan-binding lectin-associated serine proteases (MASPs). MASP-2 is considered as the autonomous pathway-activator, while MASP-1 is considered as an auxiliary component. We evolved a pair of monospecific MASP inhibitors. In accordance with the key role of MASP-2, the MASP-2 inhibitor completely blocks the lectin pathway activation. Importantly, the MASP-1 inhibitor does the same, demonstrating that MASP-1 is not an auxiliary but an essential pathway component. We report the first Michaelis-like complex structures of MASP-1 and MASP-2 formed with substrate-like inhibitors. The 1.28 Å resolution MASP-2 structure reveals significant plasticity of the protease, suggesting that either an induced fit or a conformational selection mechanism should contribute to the extreme specificity of the enzyme.
PubMed: 22511776
DOI: 10.1074/jbc.M112.354332
主引用文献が同じPDBエントリー
実験手法
X-RAY DIFFRACTION (3.2 Å)
構造検証レポート
Validation report summary of 4djz
検証レポート(詳細版)ダウンロードをダウンロード

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件を2025-07-16に公開中

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