4DEX
Crystal structure of the Voltage Dependent Calcium Channel beta-2 Subunit in Complex With The CaV2.2 I-II Linker.
Summary for 4DEX
| Entry DOI | 10.2210/pdb4dex/pdb |
| Related | 1t3l 4DEY |
| Descriptor | Voltage-dependent L-type calcium channel subunit beta-2, Voltage-dependent N-type calcium channel subunit alpha-1B (3 entities in total) |
| Functional Keywords | maguk, voltage dependent calcium channel, transport protein |
| Biological source | Oryctolagus cuniculus (European rabbit,Japanese white rabbit,domestic rabbit,rabbits) More |
| Cellular location | Cell membrane, sarcolemma ; Peripheral membrane protein ; Cytoplasmic side : P54288 Membrane; Multi-pass membrane protein: Q02294 |
| Total number of polymer chains | 2 |
| Total formula weight | 51543.75 |
| Authors | Almagor, L.,Hirsch, J.A. (deposition date: 2012-01-22, release date: 2012-06-13, Last modification date: 2024-02-28) |
| Primary citation | Almagor, L.,Chomsky-Hecht, O.,Ben-Mocha, A.,Hendin-Barak, D.,Dascal, N.,Hirsch, J.A. The role of a voltage-dependent Ca2+ channel intracellular linker: a structure-function analysis. J.Neurosci., 32:7602-7613, 2012 Cited by PubMed Abstract: Voltage-dependent calcium channels (VDCCs) allow the passage of Ca(2+) ions through cellular membranes in response to membrane depolarization. The channel pore-forming subunit, α1, and a regulatory subunit (Ca(V)β) form a high affinity complex where Ca(V)β binds to a α1 interacting domain in the intracellular linker between α1 membrane domains I and II (I-II linker). We determined crystal structures of Ca(V)β2 functional core in complex with the Ca(V)1.2 and Ca(V)2.2 I-II linkers to a resolution of 1.95 and 2.0 Å, respectively. Structural differences between the highly conserved linkers, important for coupling Ca(V)β to the channel pore, guided mechanistic functional studies. Electrophysiological measurements point to the importance of differing linker structure in both Ca(V)1 and 2 subtypes with mutations affecting both voltage- and calcium-dependent inactivation and voltage dependence of activation. These linker effects persist in the absence of Ca(V)β, pointing to the intrinsic role of the linker in VDCC function and suggesting that I-II linker structure can serve as a brake during inactivation. PubMed: 22649239DOI: 10.1523/JNEUROSCI.5727-11.2012 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2 Å) |
Structure validation
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