4DEX

Crystal structure of the Voltage Dependent Calcium Channel beta-2 Subunit in Complex With The CaV2.2 I-II Linker.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-04-02
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.873
Spacegroup name	P 1 21 1
Unit cell lengths	36.908, 68.661, 79.158
Unit cell angles	90.00, 102.55, 90.00

Refinement procedure

Resolution	27.313 - 2.000
R-factor	0.1996
Rwork	0.198
R-free	0.23810
Structure solution method	SIRAS
RMSD bond length	0.008
RMSD bond angle	1.032
Refinement software	PHENIX ((phenix.refine: 1.7.2_869))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	2.490	2.070
High resolution limit [Å]	2.000	2.400	2.000
Rmerge		0.239	0.421
Number of reflections	26222
<I/σ(I)>		2.9	1.7
Completeness [%]	90.1	55.1	53.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	292	0.1 M NaCl, 0.1 M Bicine, 23-26% PEG 400 , pH 8.0, VAPOR DIFFUSION, temperature 292K
2	VAPOR DIFFUSION	8	292	0.1 M NaCl, 0.1 M Bicine, 23-26% PEG 400 Soaked with NaBr before freezing, pH 8.0, VAPOR DIFFUSION, temperature 292K
3	VAPOR DIFFUSION	8	292	0.1 M NaCl, 0.1 M Bicine, 23-26% PEG 400 Soaked with NaBr before freezing, pH 8.0, VAPOR DIFFUSION, temperature 292K