4CU4

FhuA from E. coli in complex with the lasso peptide microcin J25 (MccJ25)

Summary for 4CU4

Entry DOI	10.2210/pdb4cu4/pdb
Related PRD ID	PRD_000184
Descriptor	FERRICHROME-IRON RECEPTOR, PHOSPHATE ION, MICROCIN J25, ... (11 entities in total)
Functional Keywords	transport protein-antibiotic complex, lipopolysaccharide, detergent, transport protein/antibiotic
Biological source	ESCHERICHIA COLI STR. K-12 SUBSTR. MG1655 More
Total number of polymer chains	2
Total formula weight	88629.36
Authors	Mathavan, I.,Rebuffat, S.,Beis, K. (deposition date: 2014-03-17, release date: 2014-04-09, Last modification date: 2024-11-13)
Primary citation	Mathavan, I.,Zirah, S.,Mehmood, S.,Choudhury, H.G.,Goulard, C.,Li, Y.,Robinson, C.V.,Rebuffat, S.,Beis, K. Structural Basis for Hijacking Siderophore Receptors by Antimicrobial Lasso Peptides. Nat.Chem.Biol., 10:340-, 2014 Cited by PubMed Abstract: The lasso peptide microcin J25 is known to hijack the siderophore receptor FhuA for initiating internalization. Here, we provide what is to our knowledge the first structural evidence on the recognition mechanism, and our biochemical data show that another closely related lasso peptide cannot interact with FhuA. Our work provides an explanation on the narrow activity spectrum of lasso peptides and opens the path to the development of new antibacterials. PubMed: 24705590 DOI: 10.1038/NCHEMBIO.1499 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.3 Å)

Structure validation