4CU4

FhuA from E. coli in complex with the lasso peptide microcin J25 (MccJ25)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	FERRICHROME-IRON RECEPTOR	polymer	706	78101.0	1	UniProt (P06971) Pfam (PF07715) Pfam (PF00593) In PDB	ESCHERICHIA COLI STR. K-12 SUBSTR. MG1655	FHUA, FERRIC HYDROXAMATE RECEPTOR, FERRIC HYDROXAMATE UPTAKE
2	B	MICROCIN J25	polymer	21	2126.3	1	UniProt (Q9X2V7) In PDB BIRD (PRD_000184)	ESCHERICHIA COLI STR. K-12 SUBSTR. MC4100	MICROCIN MCCJ25, MCCJ25
3	C	L-glycero-alpha-D-manno-heptopyranose-(1-3)-L-glycero-alpha-D-manno-heptopyranose-(1-5)-[3-deoxy-alpha-D-manno-oct-2-ulopyranosonic acid-(2-4)]3-deoxy-alpha-D-manno-oct-2-ulopyranosonic acid-(2-6)-2-amino-2-deoxy-beta-D-glucopyranose-(1-6)-2-amino-2-deoxy-alpha-D-glucopyranose	branched		1165.0	1	In PDB GlyTouCan (G23424DU)
4	A	3-HYDROXY-TETRADECANOIC ACID	non-polymer		244.4	4	Chemie (FTT)
5	A	LAURIC ACID	non-polymer		200.3	1	Chemie (DAO)
6	A	MYRISTIC ACID	non-polymer		228.4	1	Chemie (MYR)
7	A	LAURYL DIMETHYLAMINE-N-OXIDE	non-polymer		229.4	20	Chemie (LDA)
8	A	1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE	non-polymer		705.0	1	Chemie (3PH)
9	A	DIPHOSPHATE	non-polymer		173.9	2	Chemie (DPO)
10	A	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
11		water	water		18.0	168	Chemie (HOH)

Sequence modifications

A: 417 - 725 (UniProt: P06971)

PDB	External Database	Details
Ser 406	-	expression tag
Ser 407	-	expression tag
His 408	-	expression tag
His 409	-	expression tag
His 410	-	expression tag
His 411	-	expression tag
His 412	-	expression tag
His 413	-	expression tag
Gly 414	-	expression tag
Ser 415	-	expression tag
Ser 416	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	80227.3
Branched	Number of molecules	1
	Total formula weight	1165.0
Non-Polymers*	Number of molecules	31
	Total formula weight	7237.0
All*	Total formula weight	88629.4

*Water molecules are not included.