4CMQ

Crystal structure of Mn-bound S.pyogenes Cas9

Summary for 4CMQ

Entry DOI	10.2210/pdb4cmq/pdb
Related	4CMP
Descriptor	CRISPR-ASSOCIATED ENDONUCLEASE CAS9/CSN1, MANGANESE (II) ION, SULFATE ION (3 entities in total)
Functional Keywords	hydrolase, dnase, endonuclease, rna-guided, immunity, crrna, genome editing
Biological source	STREPTOCOCCUS PYOGENES
Total number of polymer chains	2
Total formula weight	319262.79
Authors	Jinek, M.,Jiang, F.,Taylor, D.W.,Sternberg, S.H.,Kaya, E.,Ma, E.,Anders, C.,Hauer, M.,Zhou, K.,Lin, S.,Kaplan, M.,Iavarone, A.T.,Charpentier, E.,Nogales, E.,Doudna, J.A. (deposition date: 2014-01-17, release date: 2014-02-12, Last modification date: 2023-12-20)
Primary citation	Jinek, M.,Jiang, F.,Taylor, D.W.,Sternberg, S.H.,Kaya, E.,Ma, E.,Anders, C.,Hauer, M.,Zhou, K.,Lin, S.,Kaplan, M.,Iavarone, A.T.,Charpentier, E.,Nogales, E.,Doudna, J.A. Structures of Cas9 Endonucleases Reveal RNA- Mediated Conformational Activation Science, 343:47997-, 2014 Cited by PubMed: 24505130 DOI: 10.1126/SCIENCE.1247997 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (3.09 Å)

Structure validation