4BWL
Structure of the Y137A mutant of E. coli N-acetylneuraminic acid lyase in complex with pyruvate, N-acetyl-D-mannosamine and N- acetylneuraminic acid
Summary for 4BWL
Entry DOI | 10.2210/pdb4bwl/pdb |
Related | 4BWK |
Descriptor | N-ACETYLNEURAMINATE LYASE, 5-(acetylamino)-3,5-dideoxy-D-glycero-D-galacto-non-2-ulosonic acid, PENTAETHYLENE GLYCOL, ... (6 entities in total) |
Functional Keywords | lyase, directed evolution, substrate specificity, protein engineering |
Biological source | ESCHERICHIA COLI More |
Cellular location | Cytoplasm: P0A6L4 P0A6L4 |
Total number of polymer chains | 4 |
Total formula weight | 135663.71 |
Authors | Campeotto, I.,Phillips, S.E.V.,Pearson, A.R.,Nelson, A.,Berry, A. (deposition date: 2013-07-03, release date: 2014-02-19, Last modification date: 2023-12-20) |
Primary citation | Daniels, A.D.,Campeotto, I.,Van Der Kamp, M.W.,Bolt, A.H.,Trinh, C.H.,Phillips, S.E.V.,Pearson, A.R.,Nelson, A.,Mulholland, A.J.,Berry, A. The Reaction Mechanism of N-Acetylneuraminic Acid Lyase Revealed by a Combination of Crystallography, Qm/Mm Simulation and Mutagenesis. Acs Chem.Biol., 9:1025-, 2014 Cited by PubMed: 24521460DOI: 10.1021/CB500067Z PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (2 Å) |
Structure validation
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