4BWL
Structure of the Y137A mutant of E. coli N-acetylneuraminic acid lyase in complex with pyruvate, N-acetyl-D-mannosamine and N- acetylneuraminic acid
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, D (A, B, D) | N-ACETYLNEURAMINATE LYASE | polymer | 304 | 33492.3 | 3 | UniProt (P0A6L4) Pfam (PF00701) | ESCHERICHIA COLI | N-ACETYLNEURAMINATE PYRUVATE-LYASE, N-ACETYLNEURAMINIC ACID ALDOLASE, NALASE, SIALATE LYASE, SIALIC ACID ALDOLASE, SIALIC ACID LYASE, N-ACETYLNEURAMINIC ACID LYASE |
| 2 | C (C) | N-ACETYLNEURAMINATE LYASE | polymer | 304 | 33561.3 | 1 | UniProt (P0A6L4) Pfam (PF00701) | ESCHERICHIA COLI | N-ACETYLNEURAMINATE PYRUVATE-LYASE, N-ACETYLNEURAMINIC ACID ALDOLASE, NALASE, SIALATE LYASE, SIALIC ACID ALDOLASE, SIALIC ACID LYASE, N-ACETYLNEURAMINIC ACID LYASE |
| 3 | E, F, J (A, B, D) | 5-(acetylamino)-3,5-dideoxy-D-glycero-D-galacto-non-2-ulosonic acid | non-polymer | 309.3 | 3 | Chemie (SI3) PubChem (51403833) PubChem (51403837) PubChem (51403839) PubChem (349962) PubChem (59691305) PubChem (51403835) PubChem (14017587) PubChem (91663215) PubChem (124922592) PubChem (124922593) PubChem (124922594) PubChem (129708853) PubChem (129708963) PubChem (135055133) | |||
| 4 | G, I (B, C) | PENTAETHYLENE GLYCOL | non-polymer | 238.3 | 2 | Chemie (1PE) PubChem (62551) | |||
| 5 | H (C) | 2-(ACETYLAMINO)-2-DEOXY-D-MANNOSE | non-polymer | 221.2 | 1 | Chemie (MN9) PubChem (20751286) PubChem (1738118) PubChem (22952041) PubChem (65150) PubChem (92164) PubChem (51403829) PubChem (44119931) PubChem (343911) PubChem (60160733) PubChem (60196345) PubChem (71684655) PubChem (71464349) PubChem (92449972) PubChem (6567190) PubChem (101043517) PubChem (12797197) PubChem (12797198) PubChem (124919571) PubChem (129630669) | |||
| 6 | K, L, M, N (A, B, C, D) | water | water | 18.0 | 102 | Chemie (HOH) |
Sequence modifications
A, B, D: 2 - 297 (UniProt: P0A6L4)
C: 2 - 297 (UniProt: P0A6L4)
| PDB | External Database | Details |
|---|---|---|
| Met -6 | - | expression tag |
| Glu -5 | - | expression tag |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| His -2 | - | expression tag |
| His -1 | - | expression tag |
| His 0 | - | expression tag |
| His 1 | - | expression tag |
| Ala 137 | Tyr 137 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -6 | - | expression tag |
| Glu -5 | - | expression tag |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| His -2 | - | expression tag |
| His -1 | - | expression tag |
| His 0 | - | expression tag |
| His 1 | - | expression tag |
| Ala 137 | Tyr 137 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 134038.1 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 1625.6 | |
| All* | Total formula weight | 135663.7 |
