4BW9

PylRS Y306G, Y384F, I405R mutant in complex with AMP-PNP

Summary for 4BW9

• Entry DOI: 10.2210/pdb4bw9/pdb
• Related: 4BWA
• Descriptor: PYRROLYSINE--TRNA LIGASE, MAGNESIUM ION, PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER, ... (6 entities in total)
• Functional Keywords: ligase
• Biological source: METHANOSARCINA MAZEI
• Cellular location: Cytoplasm (By similarity): Q8PWY1
• Total number of polymer chains: 1
• Total formula weight: 34379.49

Authors

Schneider, S.,Vrabel, M.,Gattner, M.J.,Fluegel, V.,Lopez-Carillo, V.,Carell, T. (deposition date: 2013-07-01, release date: 2013-07-31, Last modification date: 2023-12-20)

Primary citation

Schneider, S.,Gattner, M.J.,Vrabel, M.,Flugel, V.,Lopez-Carrillo, V.,Prill, S.,Carell, T.
Structural Insights Into Incorporation of Norbornene Amino Acids for Click Modification of Proteins
Chem.Bio.Chem., 14:2114-, 2013

PubMed Abstract: Three for two: by using a Methanosarcina mazei PylRS triple mutant (Y306G, Y384F, I405R) the incorporation of two new exo-norbornene-containing pyrrolysine analogues was achieved. X-ray crystallographic analysis led to the identification of the crucial structural elements involved in substrate recognition by the evolved synthetase.

PubMed: 24027216
DOI: 10.1002/CBIC.201300435

Experimental method

X-RAY DIFFRACTION (2.35 Å)