4BW9
PylRS Y306G, Y384F, I405R mutant in complex with AMP-PNP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | PYRROLYSINE--TRNA LIGASE | polymer | 291 | 33338.1 | 1 | UniProt (Q8PWY1) Pfam (PF01409) | METHANOSARCINA MAZEI | PYRROLYSINE--TRNA(PYL) LIGASE, PYRROLYSYL-TRNA SYNTHETASE, PYLRS |
| 2 | B, C (A) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 3 | D (A) | PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER | non-polymer | 506.2 | 1 | Chemie (ANP) | |||
| 4 | E (A) | PENTAETHYLENE GLYCOL | non-polymer | 238.3 | 1 | Chemie (1PE) | |||
| 5 | F, G, H, I (A) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 4 | Chemie (EDO) | |||
| 6 | J (A) | water | water | 18.0 | 44 | Chemie (HOH) |
Sequence modifications
A: 185 - 454 (UniProt: Q8PWY1)
| PDB | External Database | Details |
|---|---|---|
| Met 164 | - | expression tag |
| Gly 165 | - | expression tag |
| Ser 166 | - | expression tag |
| Ser 167 | - | expression tag |
| His 168 | - | expression tag |
| His 169 | - | expression tag |
| His 170 | - | expression tag |
| His 171 | - | expression tag |
| His 172 | - | expression tag |
| His 173 | - | expression tag |
| Ser 174 | - | expression tag |
| Ser 175 | - | expression tag |
| Gly 176 | - | expression tag |
| Leu 177 | - | expression tag |
| Val 178 | - | expression tag |
| Pro 179 | - | expression tag |
| Arg 180 | - | expression tag |
| Gly 181 | - | expression tag |
| Ser 182 | - | expression tag |
| His 183 | - | expression tag |
| Met 184 | - | expression tag |
| Gly 306 | Tyr 306 | engineered mutation |
| Phe 384 | Tyr 384 | engineered mutation |
| Arg 405 | Ile 405 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 33338.1 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 1041.4 | |
| All* | Total formula weight | 34379.5 |
