4BW9
PylRS Y306G, Y384F, I405R mutant in complex with AMP-PNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-10 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 64 |
| Unit cell lengths | 105.742, 105.742, 69.674 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.290 - 2.350 |
| R-factor | 0.17594 |
| Rwork | 0.174 |
| R-free | 0.20372 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1q7e |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.701 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.480 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.060 | 0.700 |
| Number of reflections | 18610 | |
| <I/σ(I)> | 20.1 | 3.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.4 | 8.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M SODIUM ACETATE, 15% PEG3350 |
