4AR7
X-ray structure of the cyan fluorescent protein mTurquoise
Summary for 4AR7
| Entry DOI | 10.2210/pdb4ar7/pdb |
| Related | 1B9C 1BFP 1C4F 1CV7 1EMA 1EMB 1EMC 1EME 1EMF 1EMG 1EMK 1EML 1EMM 1F09 1F0B 1GFL 1H6R 1HCJ 1HUY 1JBY 1JBZ 1JC0 1JC1 1KP5 1KYP 1KYR 1KYS 1MYW 1Q4A 1Q4B 1Q4C 1Q4D 1Q4E 1Q73 1QXT 1QY3 1QYF 1QYO 1QYQ 1RM9 1RMM 1RMO 1RMP 1RRX 1W7S 1W7T 1W7U 1YFP 1YHG 1YHH 1YHI 1YJ2 1YJF 1Z1P 1Z1Q 2AH8 2AHA 2B3P 2B3Q 2EMD 2EMN 2EMO 2FWQ 2FZU 2WSN 2WSO 2WUR 2Y0G 2YDZ 2YE0 2YE1 2YFP 3ZTF 4ANJ 4AS8 |
| Descriptor | GREEN FLUORESCENT PROTEIN (2 entities in total) |
| Functional Keywords | fluorescent protein, cfp, fret, fret donor |
| Biological source | AEQUOREA VICTORIA (JELLYFISH) |
| Total number of polymer chains | 1 |
| Total formula weight | 27724.26 |
| Authors | von Stetten, D.,Noirclerc-Savoye, M.,Goedhart, J.,Gadella, T.W.J.,Royant, A. (deposition date: 2012-04-21, release date: 2012-08-08, Last modification date: 2024-10-23) |
| Primary citation | von Stetten, D.,Noirclerc-Savoye, M.,Goedhart, J.,Gadella, T.W.J.J.,Royant, A. Structure of a Fluorescent Protein from Aequorea Victoria Bearing the Obligate-Monomer Mutation A206K. Acta Crystallogr.,Sect.F, 68:878-, 2012 Cited by PubMed Abstract: The green fluorescent protein (GFP) from the jellyfish Aequoria victoria has been shown to dimerize at high concentrations, which could lead to artefacts in imaging experiments. To ensure a truly monomeric state, an A206K mutation has been introduced into most of its widely used variants, with minimal effect on the spectroscopic properties. Here, the first structure of one of these variants, the cyan fluorescent protein mTurquoise, is presented and compared with that of its dimeric version mTurquoise-K206A. No significant structural change is detected in the chromophore cavity, reinforcing the notion that this mutation is spectroscopically silent and validating that the structural analysis performed on dimeric mutants also applies to monomeric versions. Finally, it is explained why cyan versions of GFP containing the Y66W and N146I mutations do not require the A206K mutation to prevent dimerization at high concentrations. PubMed: 22869113DOI: 10.1107/S1744309112028667 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.23 Å) |
Structure validation
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