4ABO

Mal3 CH domain homology model and mammalian tubulin (2XRP) docked into the 8.6-Angstrom cryo-EM map of Mal3-GTPgammaS-microtubules

Summary for 4ABO

• Entry DOI: 10.2210/pdb4abo/pdb
• Related: 1FFX 1IA0 1JFF 1TUB
• EMDB information: 2004 2005 2006
• Descriptor: TUBULIN BETA CHAIN, TUBULIN ALPHA-1A CHAIN, MICROTUBULE INTEGRITY PROTEIN MAL3, ... (5 entities in total)
• Functional Keywords: structural protein, cytoskeleton, gtpase, end binding
• Biological source: SCHIZOSACCHAROMYCES POMBE (FISSION YEAST); More
• Cellular location: Cytoplasm, cytoskeleton : Q10113
• Total number of polymer chains: 9
• Total formula weight: 421064.68

Authors

Maurer, S.P.,Fourniol, F.J.,Bohner, G.,Moores, C.A.,Surrey, T. (deposition date: 2011-12-09, release date: 2012-06-06, Last modification date: 2024-05-08)

Primary citation

Maurer, S.P.,Fourniol, F.J.,Bohner, G.,Moores, C.A.,Surrey, T.
Ebs Recognize a Nucleotide-Dependent Structural CAP at Growing Microtubule Ends.
Cell(Cambridge,Mass.), 149:371-, 2012

PubMed Abstract: Growing microtubule ends serve as transient binding platforms for essential proteins that regulate microtubule dynamics and their interactions with cellular substructures. End-binding proteins (EBs) autonomously recognize an extended region at growing microtubule ends with unknown structural characteristics and then recruit other factors to the dynamic end structure. Using cryo-electron microscopy, subnanometer single-particle reconstruction, and fluorescence imaging, we present a pseudoatomic model of how the calponin homology (CH) domain of the fission yeast EB Mal3 binds to the end regions of growing microtubules. The Mal3 CH domain bridges protofilaments except at the microtubule seam. By binding close to the exchangeable GTP-binding site, the CH domain is ideally positioned to sense the microtubule's nucleotide state. The same microtubule-end region is also a stabilizing structural cap protecting the microtubule from depolymerization. This insight supports a common structural link between two important biological phenomena, microtubule dynamic instability and end tracking.

PubMed: 22500803
DOI: 10.1016/J.CELL.2012.02.049

Experimental method

ELECTRON MICROSCOPY (8.6 Å)