4ABO

Mal3 CH domain homology model and mammalian tubulin (2XRP) docked into the 8.6-Angstrom cryo-EM map of Mal3-GTPgammaS-microtubules

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0000226	biological_process	microtubule cytoskeleton organization
A	0000278	biological_process	mitotic cell cycle
A	0001764	biological_process	neuron migration
A	0003924	molecular_function	GTPase activity
A	0005200	molecular_function	structural constituent of cytoskeleton
A	0005515	molecular_function	protein binding
A	0005525	molecular_function	GTP binding
A	0005737	cellular_component	cytoplasm
A	0005856	cellular_component	cytoskeleton
A	0005874	cellular_component	microtubule
A	0007017	biological_process	microtubule-based process
A	0046872	molecular_function	metal ion binding
B	0000166	molecular_function	nucleotide binding
B	0000226	biological_process	microtubule cytoskeleton organization
B	0000278	biological_process	mitotic cell cycle
B	0005200	molecular_function	structural constituent of cytoskeleton
B	0005525	molecular_function	GTP binding
B	0005737	cellular_component	cytoplasm
B	0005856	cellular_component	cytoskeleton
B	0005874	cellular_component	microtubule
B	0007017	biological_process	microtubule-based process
B	0016787	molecular_function	hydrolase activity
B	0030182	biological_process	neuron differentiation
B	0046872	molecular_function	metal ion binding
C	0000166	molecular_function	nucleotide binding
C	0000226	biological_process	microtubule cytoskeleton organization
C	0000278	biological_process	mitotic cell cycle
C	0001764	biological_process	neuron migration
C	0003924	molecular_function	GTPase activity
C	0005200	molecular_function	structural constituent of cytoskeleton
C	0005515	molecular_function	protein binding
C	0005525	molecular_function	GTP binding
C	0005737	cellular_component	cytoplasm
C	0005856	cellular_component	cytoskeleton
C	0005874	cellular_component	microtubule
C	0007017	biological_process	microtubule-based process
C	0046872	molecular_function	metal ion binding
D	0000166	molecular_function	nucleotide binding
D	0000226	biological_process	microtubule cytoskeleton organization
D	0000278	biological_process	mitotic cell cycle
D	0005200	molecular_function	structural constituent of cytoskeleton
D	0005525	molecular_function	GTP binding
D	0005737	cellular_component	cytoplasm
D	0005856	cellular_component	cytoskeleton
D	0005874	cellular_component	microtubule
D	0007017	biological_process	microtubule-based process
D	0016787	molecular_function	hydrolase activity
D	0030182	biological_process	neuron differentiation
D	0046872	molecular_function	metal ion binding
E	0000166	molecular_function	nucleotide binding
E	0000226	biological_process	microtubule cytoskeleton organization
E	0000278	biological_process	mitotic cell cycle
E	0001764	biological_process	neuron migration
E	0003924	molecular_function	GTPase activity
E	0005200	molecular_function	structural constituent of cytoskeleton
E	0005515	molecular_function	protein binding
E	0005525	molecular_function	GTP binding
E	0005737	cellular_component	cytoplasm
E	0005856	cellular_component	cytoskeleton
E	0005874	cellular_component	microtubule
E	0007017	biological_process	microtubule-based process
E	0046872	molecular_function	metal ion binding
F	0000166	molecular_function	nucleotide binding
F	0000226	biological_process	microtubule cytoskeleton organization
F	0000278	biological_process	mitotic cell cycle
F	0005200	molecular_function	structural constituent of cytoskeleton
F	0005525	molecular_function	GTP binding
F	0005737	cellular_component	cytoplasm
F	0005856	cellular_component	cytoskeleton
F	0005874	cellular_component	microtubule
F	0007017	biological_process	microtubule-based process
F	0016787	molecular_function	hydrolase activity
F	0030182	biological_process	neuron differentiation
F	0046872	molecular_function	metal ion binding
G	0000166	molecular_function	nucleotide binding
G	0000226	biological_process	microtubule cytoskeleton organization
G	0000278	biological_process	mitotic cell cycle
G	0001764	biological_process	neuron migration
G	0003924	molecular_function	GTPase activity
G	0005200	molecular_function	structural constituent of cytoskeleton
G	0005515	molecular_function	protein binding
G	0005525	molecular_function	GTP binding
G	0005737	cellular_component	cytoplasm
G	0005856	cellular_component	cytoskeleton
G	0005874	cellular_component	microtubule
G	0007017	biological_process	microtubule-based process
G	0046872	molecular_function	metal ion binding
H	0000166	molecular_function	nucleotide binding
H	0000226	biological_process	microtubule cytoskeleton organization
H	0000278	biological_process	mitotic cell cycle
H	0005200	molecular_function	structural constituent of cytoskeleton
H	0005525	molecular_function	GTP binding
H	0005737	cellular_component	cytoplasm
H	0005856	cellular_component	cytoskeleton
H	0005874	cellular_component	microtubule
H	0007017	biological_process	microtubule-based process
H	0016787	molecular_function	hydrolase activity
H	0030182	biological_process	neuron differentiation
H	0046872	molecular_function	metal ion binding
I	0008017	molecular_function	microtubule binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	19
Details	BINDING SITE FOR RESIDUE GSP C 1438

Chain	Residue
B	GLU254
C	SER140
C	GLY142
C	GLY144
C	THR145
C	GLY146
C	ASP179
C	GLU183
C	ASN206
C	TYR224
C	ASN228
C	GLY10
C	GLN11
C	CYS12
C	GLN15
C	GLU71
C	ALA99
C	GLY100
C	ASN101

---

site_id	AC2
Number of Residues	18
Details	BINDING SITE FOR RESIDUE GSP A 1438

Chain	Residue
A	GLY10
A	GLN11
A	CYS12
A	GLN15
A	GLU71
A	ALA99
A	GLY100
A	ASN101
A	SER140
A	GLY142
A	GLY144
A	THR145
A	GLY146
A	ASP179
A	GLU183
A	ASN206
A	TYR224
A	ASN228

---

site_id	AC3
Number of Residues	19
Details	BINDING SITE FOR RESIDUE GTP B 500

Chain	Residue
A	LEU248
A	LYS254
B	GLY10
B	GLN11
B	ALA12
B	GLN15
B	ALA99
B	ALA100
B	ASN101
B	SER140
B	GLY143
B	GLY144
B	THR145
B	GLY146
B	ILE171
B	GLU183
B	ASN206
B	TYR224
B	ASN228

---

site_id	AC4
Number of Residues	18
Details	BINDING SITE FOR RESIDUE GSP E 1438

Chain	Residue
E	GLY10
E	GLN11
E	CYS12
E	GLN15
E	GLU71
E	ALA99
E	GLY100
E	ASN101
E	SER140
E	GLY142
E	GLY144
E	THR145
E	GLY146
E	ASP179
E	GLU183
E	ASN206
E	TYR224
E	ASN228

---

site_id	AC5
Number of Residues	19
Details	BINDING SITE FOR RESIDUE GTP D 500

Chain	Residue
C	LEU248
C	LYS254
D	GLY10
D	GLN11
D	ALA12
D	GLN15
D	ALA99
D	ALA100
D	ASN101
D	SER140
D	GLY143
D	GLY144
D	THR145
D	GLY146
D	ILE171
D	GLU183
D	ASN206
D	TYR224
D	ASN228

---

site_id	AC6
Number of Residues	19
Details	BINDING SITE FOR RESIDUE GTP F 500

Chain	Residue
F	ALA99
F	ALA100
F	ASN101
F	SER140
F	GLY143
F	GLY144
F	THR145
F	GLY146
F	ILE171
F	GLU183
F	ASN206
F	TYR224
F	ASN228
E	LEU248
E	LYS254
F	GLY10
F	GLN11
F	ALA12
F	GLN15

---

site_id	AC7
Number of Residues	18
Details	BINDING SITE FOR RESIDUE GTP H 500

Chain	Residue
G	LEU248
H	GLY10
H	GLN11
H	ALA12
H	GLN15
H	ALA99
H	ALA100
H	ASN101
H	SER140
H	GLY143
H	GLY144
H	THR145
H	GLY146
H	ILE171
H	GLU183
H	ASN206
H	TYR224
H	ASN228

---

site_id	AC8
Number of Residues	19
Details	BINDING SITE FOR RESIDUE GSP G 1438

Chain	Residue
F	GLU254
G	GLY10
G	GLN11
G	CYS12
G	GLN15
G	GLU71
G	ALA99
G	GLY100
G	ASN101
G	SER140
G	GLY142
G	GLY144
G	THR145
G	GLY146
G	ASP179
G	GLU183
G	ASN206
G	TYR224
G	ASN228

---

Functional Information from PROSITE/UniProt

site_id	PS00227
Number of Residues	7
Details	TUBULIN Tubulin subunits alpha, beta, and gamma signature. GGGTGSG

Chain	Residue	Details
A	GLY142-GLY148
B	GLY142-GLY148

---

site_id	PS00228
Number of Residues	4
Details	TUBULIN_B_AUTOREG Tubulin-beta mRNA autoregulation signal. MREI

Chain	Residue	Details
A	MET1-ILE4

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	28
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"Q13509","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	72
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"P68363","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"P99024","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	Modified residue: {"description":"N6-succinyllysine; alternate","evidences":[{"source":"UniProtKB","id":"P99024","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	4
Details	Modified residue: {"description":"Phosphoserine; by CDK1","evidences":[{"source":"UniProtKB","id":"Q13885","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	8
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"UniProtKB","id":"P07437","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	4
Details	Modified residue: {"description":"Omega-N-methylarginine","evidences":[{"source":"UniProtKB","id":"P07437","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	4
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate","evidences":[{"source":"UniProtKB","id":"P07437","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	8
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"UniProtKB","id":"P07437","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	4
Details	Active site: {"evidences":[{"source":"UniProtKB","id":"P68363","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	4
Details	Modified residue: {"description":"3'-nitrotyrosine","evidences":[{"source":"UniProtKB","id":"P68373","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	4
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"P68373","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---