4AAD

Crystal structure of the mutant D75N I-CreI in complex with its wild- type target in absence of metal ions at the active site (The four central bases, 2NN region, are composed by GTAC from 5' to 3')

Summary for 4AAD

Related1G9Z 1BP7 2VBL 1AF5 2VBJ 2VBO 1MOW 1G9Y 1N3F 2VBN 1T9I 1U0D 1T9J 1N3E 1U0C 4AAF 4AAG 4AAB 4AAE
DescriptorDNA ENDONUCLEASE I-CREI, 24MER DNA, GLYCEROL, ... (4 entities in total)
Functional Keywordshydrolase-dna complex, gene targeting, protein-dna interaction, homing endonucleases, hydrolase/dna
Biological sourceCHLAMYDOMONAS REINHARDTII
More
Cellular locationPlastid, chloroplast P05725
Total number of polymer chains4
Total molecular weight50504.58
Authors
Molina, R.,Redondo, P.,Stella, S.,Marenchino, M.,D'Abramo, M.,Gervasio, F.L.,Epinat, J.C.,Valton, J.,Grizot, S.,Duchateau, P.,Prieto, J.,Montoya, G. (deposition date: 2011-12-01, release date: 2012-05-02, Last modification date: 2012-08-22)
Primary citation
Molina, R.,Redondo, P.,Stella, S.,Marenchino, M.,D'Abramo, M.,Gervasio, F.L.,Charles Epinat, J.,Valton, J.,Grizot, S.,Duchateau, P.,Prieto, J.,Montoya, G.
Non-Specific Protein-DNA Interactions Control I-Crei Target Binding and Cleavage.
Nucleic Acids Res., 40:6936-, 2012
PubMed: 22495931 (PDB entries with the same primary citation)
DOI: 10.1093/NAR/GKS320
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (3.1 Å)
?

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.23622013.4%0.6%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution