4AAD
Crystal structure of the mutant D75N I-CreI in complex with its wild- type target in absence of metal ions at the active site (The four central bases, 2NN region, are composed by GTAC from 5' to 3')
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | DNA ENDONUCLEASE I-CREI | polymer | 152 | 17515.1 | 2 | UniProt (P05725) Pfam (PF00961) | CHLAMYDOMONAS REINHARDTII | I-CREI, 23S RRNA INTRON PROTEIN |
| 2 | C, D (E, F) | 24MER DNA | polymer | 24 | 7368.8 | 2 | SYNTHETIC CONSTRUCT | ||
| 3 | E, F, G, H, I... (A, B, E, F) | GLYCEROL | non-polymer | 92.1 | 8 | Chemie (GOL) | |||
| 4 | M, N, O, P (A, B, E, F) | water | water | 18.0 | 26 | Chemie (HOH) |
Sequence modifications
A, B: 2 - 153 (UniProt: P05725)
| PDB | External Database | Details |
|---|---|---|
| Asn 75 | Asp 75 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 49767.8 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 736.8 | |
| All* | Total formula weight | 50504.6 |
