4AAB
Crystal structure of the mutant D75N I-CreI in complex with its wild- type target (The four central bases, 2NN region, are composed by GTAC from 5' to 3')
Summary for 4AAB
Entry DOI | 10.2210/pdb4aab/pdb |
Related | 1AF5 1BP7 1G9Y 1G9Z 1MOW 1N3E 1N3F 1T9I 1T9J 1U0C 1U0D 2VBJ 2VBL 2VBN 2VBO 4AAD 4AAE 4AAF 4AAG |
Descriptor | DNA ENDONUCLEASE I-CREI, 14MER DNA 5'-D(*TP*CP*AP*AP*AP*AP*CP*GP*TP*CP*GP*TP*AP*CP)-3', 10MER DNA 5'-D(*GP*AP*CP*GP*TP*TP*TP*TP*GP*AP)-3', ... (7 entities in total) |
Functional Keywords | hydrolase-dna complex, gene targeting, protein-dna interaction, homing endonucleases, hydrolase/dna |
Biological source | CHLAMYDOMONAS REINHARDTII More |
Cellular location | Plastid, chloroplast: P05725 |
Total number of polymer chains | 6 |
Total formula weight | 50154.29 |
Authors | Molina, R.,Redondo, P.,Stella, S.,Marenchino, M.,D'Abramo, M.,Gervasio, F.L.,Epinat, J.C.,Valton, J.,Grizot, S.,Duchateau, P.,Prieto, J.,Montoya, G. (deposition date: 2011-12-01, release date: 2012-05-02, Last modification date: 2023-12-20) |
Primary citation | Molina, R.,Redondo, P.,Stella, S.,Marenchino, M.,D'Abramo, M.,Gervasio, F.L.,Charles Epinat, J.,Valton, J.,Grizot, S.,Duchateau, P.,Prieto, J.,Montoya, G. Non-Specific Protein-DNA Interactions Control I-Crei Target Binding and Cleavage. Nucleic Acids Res., 40:6936-6945, 2012 Cited by PubMed: 22495931DOI: 10.1093/NAR/GKS320 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (2.5 Å) |
Structure validation
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