4AAB
Crystal structure of the mutant D75N I-CreI in complex with its wild- type target (The four central bases, 2NN region, are composed by GTAC from 5' to 3')
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B | DNA ENDONUCLEASE I-CREI | polymer | 152 | 17515.1 | 2 | UniProt (P05725) Pfam (PF00961) In PDB | CHLAMYDOMONAS REINHARDTII | 23S RRNA INTRON PROTEIN, I-CREI |
| 2 | D, F | 14MER DNA 5'-D(*TP*CP*AP*AP*AP*AP*CP*GP*TP*CP*GP*TP*AP*CP)-3' | polymer | 14 | 4248.8 | 2 | SYNTHETIC CONSTRUCT | ||
| 3 | E, G | 10MER DNA 5'-D(*GP*AP*CP*GP*TP*TP*TP*TP*GP*AP)-3' | polymer | 10 | 3075.0 | 2 | SYNTHETIC CONSTRUCT | ||
| 4 | B, D, E | S-1,2-PROPANEDIOL | non-polymer | 76.1 | 5 | Chemie (PGO) | |||
| 5 | B, E | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) | |||
| 6 | G | SODIUM ION | non-polymer | 23.0 | 1 | Chemie (NA) | |||
| 7 | water | water | 18.0 | 122 | Chemie (HOH) |
Sequence modifications
A, B: 2 - 153 (UniProt: P05725)
| PDB | External Database | Details |
|---|---|---|
| Asn 75 | Asp 75 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 49677.9 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 476.4 | |
| All* | Total formula weight | 50154.3 |
