3ZJY

Crystal Structure of Importin 13 - RanGTP - eIF1A complex

Summary for 3ZJY

• Entry DOI: 10.2210/pdb3zjy/pdb
• Related: 3ZKV
• Descriptor: GTP-BINDING NUCLEAR PROTEIN RAN, IMPORTIN-13, EUKARYOTIC TRANSLATION INITIATION FACTOR 1A, X-CHROMOSOMAL, ... (5 entities in total)
• Functional Keywords: translation, active transport, cell nucleus, nucleocytoplasmic transport, multiprotein complex, rna-binding protein, ran gtp-binding protein
• Biological source: HOMO SAPIENS (HUMAN); More
• Cellular location: Nucleus: P62826; Cytoplasm: O94829
• Total number of polymer chains: 7
• Total formula weight: 401252.45

Authors

Gruenwald, M.,Lazzaretti, D.,Bono, F. (deposition date: 2013-01-21, release date: 2013-04-10, Last modification date: 2023-12-20)

Primary citation

Grunwald, M.,Lazzaretti, D.,Bono, F.
Structural Basis for the Nuclear Export Activity of Importin13.
Embo J., 32:899-, 2013

PubMed Abstract: Importin13 (Imp13) is a bidirectional karyopherin that can mediate both import and export of cargoes. Imp13 recognizes several import cargoes, which include the exon junction complex components Mago-Y14 and the E2 SUMO-conjugating enzyme Ubc9, and one known export cargo, the translation initiation factor 1A (eIF1A). To understand how Imp13 can perform double duty, we determined the 3.6-Å crystal structure of Imp13 in complex with RanGTP and with eIF1A. eIF1A binds at the inner surface of the Imp13 C-terminal arch adjacent and concomitantly to RanGTP illustrating how eIF1A can be exported by Imp13. Moreover, the 3.0-Å structure of Imp13 in its unbound state reveals the existence of an open conformation in the cytoplasm that explains export cargo release and completes the export branch of the Imp13 pathway. Finally, we demonstrate that Imp13 is able to bind and export eIF1A in vivo and that its function is essential.

PubMed: 23435562
DOI: 10.1038/EMBOJ.2013.29

Experimental method

X-RAY DIFFRACTION (3.6 Å)