3ZBO

A new family of proteins related to the HEAT-like repeat DNA glycosylases with affinity for branched DNA structures

Summary for 3ZBO

• Entry DOI: 10.2210/pdb3zbo/pdb
• Descriptor: ALKF, CHLORIDE ION (3 entities in total)
• Functional Keywords: unknown function, dna damage, base excision repair, alkc, alkd, holliday junctions
• Biological source: BACILLUS CEREUS
• Total number of polymer chains: 2
• Total formula weight: 54203.40

Authors

Backe, P.H.,Simm, R.,Laerdahl, J.K.,Dalhus, B.,Fagerlund, A.,Okstad, O.A.,Rognes, T.,Alseth, I.,Kolsto, A.-B.,Bjoras, M. (deposition date: 2012-11-12, release date: 2013-05-15, Last modification date: 2023-12-20)

Primary citation

Backe, P.H.,Simm, R.,Laerdahl, J.K.,Dalhus, B.,Fagerlund, A.,Okstad, O.A.,Rognes, T.,Alseth, I.,Kolsto, A.-B.,Bjoras, M.
A New Family of Proteins Related to the Heat-Like Repeat DNA Glycosylases with Affinity for Branched DNA Structures.
J.Struct.Biol., 183:66-, 2013

PubMed Abstract: The recently discovered HEAT-like repeat (HLR) DNA glycosylase superfamily is widely distributed in all domains of life. The present bioinformatics and phylogenetic analysis shows that HLR DNA glycosylase superfamily members in the genus Bacillus form three subfamilies: AlkC, AlkD and AlkF/AlkG. The crystal structure of AlkF shows structural similarity with the DNA glycosylases AlkC and AlkD, however neither AlkF nor AlkG display any DNA glycosylase activity. Instead, both proteins have affinity to branched DNA structures such as three-way and Holliday junctions. A unique β-hairpin in the AlkF/AlkG subfamily is most likely inserted into the DNA major groove, and could be a structural determinant regulating DNA substrate affinity. We conclude that AlkF and AlkG represent a new family of HLR proteins with affinity for branched DNA structures.

PubMed: 23623903
DOI: 10.1016/J.JSB.2013.04.007

Experimental method

X-RAY DIFFRACTION (1.58 Å)