3ZBO
A new family of proteins related to the HEAT-like repeat DNA glycosylases with affinity for branched DNA structures
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH SX-165 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.921, 87.159, 112.604 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.470 - 1.580 |
| R-factor | 0.16692 |
| Rwork | 0.165 |
| R-free | 0.20452 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1t06 |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.263 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.640 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.050 | 0.310 |
| Number of reflections | 68454 | |
| <I/σ(I)> | 25.97 | 3.76 |
| Completeness [%] | 99.6 | 98.5 |
| Redundancy | 3.9 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
