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3WZO

Crystal structure of the core streptavidin mutant V21 (Y22S/N23D/S27D/Y83S/R84K/E101D/R103K/E116N) complexed with biotin long tail (BTNtail) at 1.5 A resolution

Summary for 3WZO
Entry DOI10.2210/pdb3wzo/pdb
Related3WZN 3WZP 3WZQ
DescriptorStreptavidin, 6-({5-[(3aS,4S,5S,6aR)-5-oxido-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoyl}amino)hexanoic acid, GLYCEROL, ... (6 entities in total)
Functional Keywordsbeta-barrel, biotin binding protein
Biological sourceStreptomyces avidinii
Cellular locationSecreted: P22629
Total number of polymer chains4
Total formula weight56255.47
Authors
Kawato, T.,Mizohata, E.,Shimizu, Y.,Meshizuka, T.,Yamamoto, T.,Takasu, N.,Matsuoka, M.,Matsumura, H.,Tsumoto, K.,Kodama, T.,Kanai, M.,Doi, H.,Inoue, T.,Sugiyama, A. (deposition date: 2014-10-01, release date: 2015-02-18, Last modification date: 2023-11-08)
Primary citationKawato, T.,Mizohata, E.,Shimizu, Y.,Meshizuka, T.,Yamamoto, T.,Takasu, N.,Matsuoka, M.,Matsumura, H.,Kodama, T.,Kanai, M.,Doi, H.,Inoue, T.,Sugiyama, A.
Structure-based design of a streptavidin mutant specific for an artificial biotin analogue.
J.Biochem., 157:467-475, 2015
Cited by
PubMed: 25645976
DOI: 10.1093/jb/mvv004
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (1.5 Å)
Structure validation

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