3WZO

Crystal structure of the core streptavidin mutant V21 (Y22S/N23D/S27D/Y83S/R84K/E101D/R103K/E116N) complexed with biotin long tail (BTNtail) at 1.5 A resolution

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0009374	molecular_function	biotin binding
B	0009374	molecular_function	biotin binding
C	0009374	molecular_function	biotin binding
D	0009374	molecular_function	biotin binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	15
Details	BINDING SITE FOR RESIDUE ZOE A 201

Chain	Residue
A	ASP27
A	TRP108
A	LEU110
A	ASP128
C	CD203
C	HOH331
D	LYS121
A	TYR43
A	SER45
A	VAL47
A	GLY48
A	ASN49
A	TRP79
A	SER88
A	THR90

---

site_id	AC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE GOL A 202

Chain	Residue
A	GLU14
A	ALA35
A	ASP36
A	TYR60
A	HOH322
C	ARG53

---

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CD A 203

Chain	Residue
A	ASP101
A	HOH333
A	HOH334
C	GLU44

---

site_id	AC4
Number of Residues	3
Details	BINDING SITE FOR RESIDUE CD A 204

Chain	Residue
A	GLU14
A	SER62
A	HOH337

---

site_id	AC5
Number of Residues	2
Details	BINDING SITE FOR RESIDUE CD A 205

Chain	Residue
A	GLU44
A	HOH356

---

site_id	AC6
Number of Residues	9
Details	BINDING SITE FOR RESIDUE P6G A 206

Chain	Residue
A	SER69
A	GLY70
A	THR71
A	TYR96
A	GLY98
A	GLY99
A	ALA100
C	ASP23
C	GLY26

---

site_id	AC7
Number of Residues	14
Details	BINDING SITE FOR RESIDUE ZOE B 201

Chain	Residue
B	ASP27
B	TYR43
B	SER45
B	VAL47
B	ASN49
B	TRP79
B	SER88
B	THR90
B	TRP108
B	LEU110
B	ASP128
C	TRP120
D	CD203
D	HOH353

---

site_id	AC8
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CD B 202

Chain	Residue
B	GLU14
B	SER62
B	HOH325
B	HOH331

---

site_id	AC9
Number of Residues	13
Details	BINDING SITE FOR RESIDUE ZOE C 201

Chain	Residue
B	TRP120
B	LYS121
C	ASP27
C	TYR43
C	SER45
C	VAL47
C	ASN49
C	TRP79
C	SER88
C	THR90
C	TRP108
C	LEU110
C	ASP128

---

site_id	BC1
Number of Residues	7
Details	BINDING SITE FOR RESIDUE GOL C 202

Chain	Residue
A	THR32
A	ALA35
A	THR40
A	HOH362
C	ILE30
C	GLY41
C	THR42

---

site_id	BC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CD C 203

Chain	Residue
A	ZOE201
C	HIS87
C	HOH331
D	HOH332

---

site_id	BC3
Number of Residues	14
Details	BINDING SITE FOR RESIDUE ZOE D 201

Chain	Residue
A	TRP120
D	ASP27
D	TYR43
D	SER45
D	VAL47
D	GLY48
D	ASN49
D	TRP79
D	SER88
D	THR90
D	TRP108
D	LEU110
D	SER112
D	ASP128

---

site_id	BC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE GOL D 202

Chain	Residue
D	TYR60
D	GLU14
D	GLY34
D	ALA35
D	ASP36

---

site_id	BC5
Number of Residues	5
Details	BINDING SITE FOR RESIDUE CD D 203

Chain	Residue
B	ZOE201
D	HIS87
D	HOH326
D	HOH343
D	HOH353

---

site_id	BC6
Number of Residues	3
Details	BINDING SITE FOR RESIDUE CD D 204

Chain	Residue
D	GLU14
D	SER62
D	HOH327

---

site_id	BC7
Number of Residues	2
Details	BINDING SITE FOR RESIDUE CD D 205

Chain	Residue
A	ASP67
D	GLU44

---

site_id	BC8
Number of Residues	4
Details	BINDING SITE FOR RESIDUE CD D 206

Chain	Residue
B	GLU44
D	ASP101
D	HOH335
D	HOH344

---

site_id	BC9
Number of Residues	7
Details	BINDING SITE FOR RESIDUE P6G D 207

Chain	Residue
B	SER22
B	ASP23
B	GLY26
D	THR71
D	TYR96
D	GLY98
D	GLY99

---

Functional Information from PROSITE/UniProt

site_id	PS00577
Number of Residues	15
Details	AVIDIN_1 Avidin-like domain signature. NawKStlVGhDtFTK

Chain	Residue	Details
A	ASN118-LYS132

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	366
Details	Domain: {"description":"Avidin-like","evidences":[{"source":"PROSITE-ProRule","id":"PRU00656","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	8
Details	Motif: {"description":"Cell attachment site; atypical"}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	20
Details	Binding site: {}

Chain

Residue

Details

---