3WZO
Crystal structure of the core streptavidin mutant V21 (Y22S/N23D/S27D/Y83S/R84K/E101D/R103K/E116N) complexed with biotin long tail (BTNtail) at 1.5 A resolution
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Streptavidin | polymer | 129 | 13227.2 | 4 | UniProt (P22629) Pfam (PF01382) | Streptomyces avidinii | |
| 2 | E, K, M, P (A, B, C, D) | 6-({5-[(3aS,4S,5S,6aR)-5-oxido-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoyl}amino)hexanoic acid | non-polymer | 373.5 | 4 | Chemie (ZOE) | |||
| 3 | F, N, Q (A, C, D) | GLYCEROL | non-polymer | 92.1 | 3 | Chemie (GOL) | |||
| 4 | G, H, I, L, O... (A, B, C, D) | CADMIUM ION | non-polymer | 112.4 | 9 | Chemie (CD) | |||
| 5 | J, V (A, D) | HEXAETHYLENE GLYCOL | non-polymer | 282.3 | 2 | Chemie (P6G) | |||
| 6 | W, X, Y, Z (A, B, C, D) | water | water | 18.0 | 233 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 13 - 139 (UniProt: P22629)
| PDB | External Database | Details |
|---|---|---|
| Gly 11 | - | expression tag |
| Pro 12 | - | expression tag |
| Ser 22 | Tyr 46 | engineered mutation |
| Asp 23 | Asn 47 | engineered mutation |
| Asp 27 | Ser 51 | engineered mutation |
| Ser 83 | Tyr 107 | engineered mutation |
| Lys 84 | Arg 108 | engineered mutation |
| Asp 101 | Glu 125 | engineered mutation |
| Lys 103 | Arg 127 | engineered mutation |
| Asn 116 | Glu 140 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 52909.0 | |
| Non-Polymers* | Number of molecules | 18 |
| Total formula weight | 3346.5 | |
| All* | Total formula weight | 56255.5 |
