3WXQ

Serial femtosecond X-ray structure of human fatty acid-binding protein type-3 (FABP3) in complex with stearic acid (C18:0) determined using X-ray free-electron laser at SACLA

Summary for 3WXQ

• Entry DOI: 10.2210/pdb3wxq/pdb
• Related: 3WUL 3WUM 3WVM 3WXQ 3WXS 3WXT 3WXU 4W4Q
• Descriptor: Fatty acid-binding protein, heart, STEARIC ACID (3 entities in total)
• Functional Keywords: antiparallel beta barrel, intracellular transport, heart, transport protein
• Biological source: Homo sapiens (human)
• Cellular location: Cytoplasm: P05413
• Total number of polymer chains: 1
• Total formula weight: 15163.50

Authors

Mizohata, E.,Suzuki, M.,Kakinouchi, K.,Sugiyama, S.,Murata, M.,Sugahara, M.,Nango, E.,Tanaka, T.,Tanaka, R.,Tono, K.,Song, C.,Hatsui, T.,Joti, Y.,Yabashi, M.,Iwata, S. (deposition date: 2014-08-04, release date: 2014-11-05, Last modification date: 2023-11-08)

Primary citation

Sugahara, M.,Mizohata, E.,Nango, E.,Suzuki, M.,Tanaka, T.,Masuda, T.,Tanaka, R.,Shimamura, T.,Tanaka, Y.,Suno, C.,Ihara, K.,Pan, D.,Kakinouchi, K.,Sugiyama, S.,Murata, M.,Inoue, T.,Tono, K.,Song, C.,Park, J.,Kameshima, T.,Hatsui, T.,Joti, Y.,Yabashi, M.,Iwata, S.
Grease matrix as a versatile carrier of proteins for serial crystallography
Nat. Methods, 12:61-63, 2015

PubMed Abstract: Serial femtosecond X-ray crystallography (SFX) has revolutionized atomic-resolution structural investigation by expanding applicability to micrometer-sized protein crystals, even at room temperature, and by enabling dynamics studies. However, reliable crystal-carrying media for SFX are lacking. Here we introduce a grease-matrix carrier for protein microcrystals and obtain the structures of lysozyme, glucose isomerase, thaumatin and fatty acid-binding protein type 3 under ambient conditions at a resolution of or finer than 2 Å.

PubMed: 25384243
DOI: 10.1038/nmeth.3172

Experimental method

X-RAY DIFFRACTION (1.6 Å)