3WX1

Mouse Cereblon thalidomide binding domain, selenomethionine derivative

Summary for 3WX1

• Entry DOI: 10.2210/pdb3wx1/pdb
• Related: 3wx2
• Descriptor: Protein cereblon, ZINC ION, SULFATE ION, ... (4 entities in total)
• Functional Keywords: zinc finger, e3 ubiquitin ligase, metal binding protein
• Biological source: Mus musculus (mouse)
• Cellular location: Cytoplasm (By similarity): Q8C7D2
• Total number of polymer chains: 2
• Total formula weight: 25373.19

Authors

Mori, T.,Ito, T.,Hirano, Y.,Yamaguchi, Y.,Handa, H.,Hakoshima, T. (deposition date: 2014-07-10, release date: 2014-08-06, Last modification date: 2024-10-30)

Primary citation

Chamberlain, P.P.,Lopez-Girona, A.,Miller, K.,Carmel, G.,Pagarigan, B.,Chie-Leon, B.,Rychak, E.,Corral, L.G.,Ren, Y.J.,Wang, M.,Riley, M.,Delker, S.L.,Ito, T.,Ando, H.,Mori, T.,Hirano, Y.,Handa, H.,Hakoshima, T.,Daniel, T.O.,Cathers, B.E.
Structure of the human Cereblon-DDB1-lenalidomide complex reveals basis for responsiveness to thalidomide analogs
Nat.Struct.Mol.Biol., 21:803-809, 2014

PubMed Abstract: The Cul4-Rbx1-DDB1-Cereblon E3 ubiquitin ligase complex is the target of thalidomide, lenalidomide and pomalidomide, therapeutically important drugs for multiple myeloma and other B-cell malignancies. These drugs directly bind Cereblon (CRBN) and promote the recruitment of substrates Ikaros (IKZF1) and Aiolos (IKZF3) to the E3 complex, thus leading to substrate ubiquitination and degradation. Here we present the crystal structure of human CRBN bound to DDB1 and the drug lenalidomide. A hydrophobic pocket in the thalidomide-binding domain (TBD) of CRBN accommodates the glutarimide moiety of lenalidomide, whereas the isoindolinone ring is exposed to solvent. We also solved the structures of the mouse TBD in the apo state and with thalidomide or pomalidomide. Site-directed mutagenesis in lentiviral-expression myeloma models showed that key drug-binding residues are critical for antiproliferative effects.

PubMed: 25108355
DOI: 10.1038/nsmb.2874

Experimental method

X-RAY DIFFRACTION (1.93 Å)