3WX1
Mouse Cereblon thalidomide binding domain, selenomethionine derivative
Experimental procedure
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-01-26 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.97911, 0.99513, 0.97941 |
Spacegroup name | I 21 3 |
Unit cell lengths | 118.112, 118.112, 118.112 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.100 - 1.930 |
R-factor | 0.19057 |
Rwork | 0.189 |
R-free | 0.21224 |
Structure solution method | MAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.993 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.930 |
Number of reflections | 20774 |
Completeness [%] | 100.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.7 | 277 | 0.1M sodium acetate (pH 5.7), 0.2M sodium sulphate, 12% PEG3350, 30% glycerol, VAPOR DIFFUSION, temperature 277K |