3V6M

Inhibition of caspase-6 activity by single mutation outside the active site

3V6M の概要

• エントリーDOI: 10.2210/pdb3v6m/pdb
• 関連するPDBエントリー: 3NR2 3OD5 3V6L
• 分子名称: Caspase-6 (2 entities in total)
• 機能のキーワード: caspase domain, hydrolase
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm: P55212
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 257822.92

構造登録者

Cao, Q.,Wang, X.J.,Liu, D.F.,Li, L.F.,Su, X.D. (登録日: 2011-12-20, 公開日: 2012-03-28, 最終更新日: 2023-11-08)

主引用文献

Cao, Q.,Wang, X.J.,Liu, C.W.,Liu, D.F.,Li, L.F.,Gao, Y.Q.,Su, X.D.
Inhibitory mechanism of caspase-6 phosphorylation revealed by crystal structures, molecular dynamics simulations, and biochemical assays
J.Biol.Chem., 287:15371-15379, 2012

PubMed Abstract: The apoptotic effector caspase-6 (CASP6) has been clearly identified as a drug target due to its strong association with neurodegeneration and axonal pruning events as well as its crucial roles in Huntington disease and Alzheimer disease. CASP6 activity is suppressed by ARK5-mediated phosphorylation at Ser(257) with an unclear mechanism. In this work, we solved crystal structures of ΔproCASP6S257E and p20/p10S257E, which mimicked the phosphorylated CASP6 zymogen and activated CASP6, respectively. The structural investigation combined with extensive biochemical assay and molecular dynamics simulation studies revealed that phosphorylation on Ser(257) inhibited self-activation of CASP6 zymogen by "locking" the enzyme in the TEVD(193)-bound "inhibited state." The structural and biochemical results also showed that phosphorylation on Ser(257) inhibited the CASP6 activity by steric hindrance. These results disclosed the inhibition mechanism of CASP6 phosphorylation and laid the foundation for a new strategy of rational CASP6 drug design.

PubMed: 22433863
DOI: 10.1074/jbc.M112.351213

実験手法

X-RAY DIFFRACTION (2.692 Å)