3UKM

Crystal structure of the human two pore domain potassium ion channel K2P1 (TWIK-1)

3UKM の概要

• エントリーDOI: 10.2210/pdb3ukm/pdb
• 分子名称: Potassium channel subfamily K member 1, UNDECANE, POTASSIUM ION, ... (4 entities in total)
• 機能のキーワード: potassium channel, membrane protein, eukaryotic, two-pore domain potassium channel, k2p channel, membrane
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Membrane; Multi-pass membrane protein (Potential): O00180
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 128660.16

構造登録者

Long, S.B.,Miller, A.N. (登録日: 2011-11-09, 公開日: 2012-02-08, 最終更新日: 2024-10-30)

主引用文献

Miller, A.N.,Long, S.B.
Crystal structure of the human two-pore domain potassium channel K2P1.
Science, 335:432-436, 2012

PubMed Abstract: Two-pore domain potassium (K(+)) channels (K2P channels) control the negative resting potential of eukaryotic cells and regulate cell excitability by conducting K(+) ions across the plasma membrane. Here, we present the 3.4 angstrom resolution crystal structure of a human K2P channel, K2P1 (TWIK-1). Unlike other K(+) channel structures, K2P1 is dimeric. An extracellular cap domain located above the selectivity filter forms an ion pathway in which K(+) ions flow through side portals. Openings within the transmembrane region expose the pore to the lipid bilayer and are filled with electron density attributable to alkyl chains. An interfacial helix appears structurally poised to affect gating. The structure lays a foundation to further investigate how K2P channels are regulated by diverse stimuli.

PubMed: 22282804
DOI: 10.1126/science.1213274

実験手法

X-RAY DIFFRACTION (3.4 Å)