3UKM
Crystal structure of the human two pore domain potassium ion channel K2P1 (TWIK-1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-05-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.080, 123.171, 119.305 |
| Unit cell angles | 90.00, 94.91, 90.00 |
Refinement procedure
| Resolution | 19.910 - 3.400 |
| R-factor | 0.273 |
| Rwork | 0.273 |
| R-free | 0.27900 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.460 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Number of reflections | 32855 | |
| <I/σ(I)> | 38.5 | 2.1 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 12.8 | 12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 20% Peg 400, 150 mM KCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
