3TM4

Crystal structure of Trm14 from Pyrococcus furiosus in complex with S-adenosylmethionine

Summary for 3TM4

Related3TLJ 3TMA 3TM5
DescriptortRNA (guanine N2-)-methyltransferase Trm14, S-ADENOSYLMETHIONINE (3 entities in total)
Functional Keywordsrossmann fold, methyltransferase, thump domain, trna methyltransferase, transferase
Biological sourcePyrococcus furiosus
Total number of polymer chains2
Total molecular weight85060.26
Authors
Fislage, M.,Roovers, M.,Tuszynska, I.,Bujnicki, J.M.,Droogmans, L.,Versees, W. (deposition date: 2011-08-31, release date: 2012-03-14, Last modification date: 2012-10-03)
Primary citation
Fislage, M.,Roovers, M.,Tuszynska, I.,Bujnicki, J.M.,Droogmans, L.,Versees, W.
Crystal structures of the tRNA:m2G6 methyltransferase Trm14/TrmN from two domains of life.
Nucleic Acids Res., 40:5149-5161, 2012
PubMed: 22362751 (PDB entries with the same primary citation)
DOI: 10.1093/nar/gks163
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (1.95 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.18860.3%0.2%1.6%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 3tm4
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Molmil generated image of 3tm4
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Molmil generated image of 3tm4
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More Biological unit images

Molmil generated image of 3tm4
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Molmil generated image of 3tm4
rotated about x axis by 90°
Molmil generated image of 3tm4
rotated about y axis by 90°
(*)In the case of coarse surface representation, the asymmetric unit is shown as red ribbon representation.
Coordinate files for Biological unit (3tm4.pdb1.gz [66.02 KB])
Coordinate files for Biological unit (3tm4.pdb2.gz [65.18 KB])