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3TM4

Crystal structure of Trm14 from Pyrococcus furiosus in complex with S-adenosylmethionine

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSLS BEAMLINE X06DA
Synchrotron siteSLS
BeamlineX06DA
Temperature [K]100
Detector technologyCCD
Collection date2011-02-25
DetectorMARMOSAIC 225 mm CCD
Wavelength(s)1.00150
Spacegroup nameP 1 21 1
Unit cell lengths82.080, 45.170, 121.430
Unit cell angles90.00, 91.79, 90.00
Refinement procedure
Resolution49.530 - 1.950
R-factor0.18258
Rwork0.182
R-free0.19592
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)3tlj
RMSD bond length0.015
RMSD bond angle1.400
Data reduction softwareXDS
Data scaling softwareXDS
Phasing softwarePHASER
Refinement softwareREFMAC (5.5.0109)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.000
High resolution limit [Å]1.9501.950
Rmerge0.0730.544
Number of reflections65283
<I/σ(I)>18.293.6
Completeness [%]99.598.9
Redundancy7.16.6
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP8293100 mM Tris-acetate, 32% PEG 4000, 15% glycerol, crystal was soaked in crystallization solution containing 1 mM S-adenosylmethionine, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 293K

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