3SFM

Novel crystallization conditions for tandem variant R67 DHFR yields wild-type crystal structure

Summary for 3SFM

Entry DOI	10.2210/pdb3sfm/pdb
Descriptor	Dihydrofolate reductase type 2, (4R)-2-METHYLPENTANE-2,4-DIOL (3 entities in total)
Functional Keywords	oxidoreductase, dihydrofolate reductase, antibiotic resistance, in situ proteolysis, type ii dhfr, sh3, reductase, dhf and nadph-binding
Biological source	Escherichia coli
Total number of polymer chains	1
Total formula weight	7088.03
Authors	Yachnin, B.J.,Berghuis, A.M. (deposition date: 2011-06-13, release date: 2011-11-02, Last modification date: 2023-09-13)
Primary citation	Yachnin, B.J.,Colin, D.Y.,Volpato, J.P.,Ebert, M.,Pelletier, J.N.,Berghuis, A.M. Novel crystallization conditions for tandem variant R67 DHFR yield a wild-type crystal structure. Acta Crystallogr.,Sect.F, 67:1316-1322, 2011 Cited by PubMed: 22102224 DOI: 10.1107/S1744309111030417 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.4 Å)

Structure validation