3SFM

Novel crystallization conditions for tandem variant R67 DHFR yields wild-type crystal structure

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004146	molecular_function	dihydrofolate reductase activity
A	0009410	biological_process	response to xenobiotic stimulus

Functional Information from PDB Data

site_id	AC1
Number of Residues	3
Details	BINDING SITE FOR RESIDUE MRD A 400

Chain	Residue
A	ALA22
A	ARG76
A	HOH109

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site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MRD A 401

Chain	Residue
A	ALA22
A	PHE24
A	MET26
A	TRP45

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site_id	AC3
Number of Residues	2
Details	BINDING SITE FOR RESIDUE MRD A 402

Chain	Residue
A	GLY35
A	LEU50

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	7
Details	Binding site: {"evidences":[{"source":"PubMed","id":"17473013","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"18052202","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"2P4T","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2RK1","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2RK2","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI2
Number of Residues	1
Details	Binding site: {"evidences":[{"source":"PubMed","id":"18052202","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"2RK1","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	4
Details	M-CSA 752

Chain	Residue	Details
A	LYS32	electrostatic stabiliser
A	GLN67	electrostatic stabiliser
A	ILE68	electrostatic stabiliser
A	TYR69	electrostatic stabiliser

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