3RDO

Crystal structure of R7-2 streptavidin complexed with biotin

Summary for 3RDO

• Entry DOI: 10.2210/pdb3rdo/pdb
• Related: 3RDM 3RDQ 3RDS 3RDU 3RDX 3RE5 3RE6
• Descriptor: Streptavidin, BIOTIN, NICKEL (II) ION, ... (6 entities in total)
• Functional Keywords: streptavidin variants, improved desthiobiotin binding, opened loop destabilization, biotin binding protein
• Biological source: Streptomyces avidinii
• Cellular location: Secreted: P22629
• Total number of polymer chains: 1
• Total formula weight: 16549.64

Authors

Malashkevich, V.N.,Magalhaes, M.,Czecster, C.M.,Guan, R.,Levy, M.,Almo, S.C. (deposition date: 2011-04-01, release date: 2011-07-06, Last modification date: 2023-09-13)

• Primary citation: Magalhaes, M.L.,Czekster, C.M.,Guan, R.,Malashkevich, V.N.,Almo, S.C.,Levy, M.; Evolved streptavidin mutants reveal key role of loop residue in high-affinity binding.; Protein Sci., 20:1145-1154, 2011; PubMed: 21520321; DOI: 10.1002/pro.642

Experimental method

X-RAY DIFFRACTION (1.404 Å)