3RDO

Crystal structure of R7-2 streptavidin complexed with biotin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Streptavidin	polymer	153	15966.4	1	UniProt (P22629) Pfam (PF01382) In PDB	Streptomyces avidinii
2	A	BIOTIN	non-polymer		244.3	1	Chemie (BTN)
3	A	NICKEL (II) ION	non-polymer		58.7	1	Chemie (NI)
4	A	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
5	A	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
6		water	water		18.0	193	Chemie (HOH)

Sequence modifications

A: 13 - 140 (UniProt: P22629)

PDB	External Database	Details
Met -12	-	expression tag
Ser -11	-	expression tag
Gly -10	-	expression tag
Ser -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Ser -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag
Ile 1	-	expression tag
Glu 2	-	expression tag
Gly 3	-	expression tag
Arg 4	-	expression tag
Gly 5	-	expression tag
Arg 6	-	expression tag
Leu 7	-	expression tag
Ile 8	-	expression tag
Lys 9	-	expression tag
His 10	-	expression tag
Met 11	-	expression tag
Thr 12	-	expression tag
Leu 29	Phe 53	engineered mutation
Gly 52	Ser 76	engineered mutation
Ser 53	Arg 77	engineered mutation
Ser 90	Thr 114	engineered mutation
Val 108	Trp 132	engineered mutation
Thr 110	Leu 134	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	15966.4
Non-Polymers*	Number of molecules	5
	Total formula weight	583.3
All*	Total formula weight	16549.6

*Water molecules are not included.