3RDQ

Crystal structure of R7-2 streptavidin complexed with desthiobiotin

Summary for 3RDQ

Entry DOI	10.2210/pdb3rdq/pdb
Related	3RDM 3RDO 3RDS 3RDU 3RDX 3RE5 3RE6
Descriptor	Streptavidin, 6-(5-METHYL-2-OXO-IMIDAZOLIDIN-4-YL)-HEXANOIC ACID, NICKEL (II) ION, ... (6 entities in total)
Functional Keywords	streptavidin variants, improved desthiobiotin binding, opened loop destabilization, biotin binding protein
Biological source	Streptomyces avidinii
Cellular location	Secreted: P22629
Total number of polymer chains	1
Total formula weight	16446.51
Authors	Malashkevich, V.N.,Magalhaes, M.,Czecster, C.M.,Guan, R.,Levy, M.,Almo, S.C. (deposition date: 2011-04-01, release date: 2011-07-06, Last modification date: 2023-09-13)
Primary citation	Magalhaes, M.L.,Czekster, C.M.,Guan, R.,Malashkevich, V.N.,Almo, S.C.,Levy, M. Evolved streptavidin mutants reveal key role of loop residue in high-affinity binding. Protein Sci., 20:1145-1154, 2011 Cited by PubMed: 21520321 DOI: 10.1002/pro.642 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.6 Å)

Structure validation