Crystal Structure of Human Flap Endonuclease FEN1 (WT) in complex with product 5'-flap DNA, SM3+, and K+

Summary for 3Q8K

Related3Q8L 3Q8M
DescriptorFlap endonuclease 1, DNA (5'-D(*AP*CP*TP*CP*TP*GP*CP*CP*TP*CP*AP*AP*GP*AP*CP*GP*GP*T)-3'), DNA (5'-D(P*TP*GP*AP*GP*GP*CP*AP*GP*AP*GP*T)-3'), ... (8 entities in total)
Functional Keywordshelix-3 turn-helix, hydrophobic wedge, 3' flap binding site, hydrolase-dna complex, dna repair, replication, flap endonuclease, fen, fen1, dna, nuclease, 5' flap, ss-dsdna junction, helix-2 turn-helix, h2th, h3th, divalent cation, helical gateway, cap, acid block, two metal mechanism, unpaired, 5' nuclease, human, long patch base excision repair, hydrolase/dna
Biological sourceHomo sapiens (human)
Cellular locationNucleus, nucleolus P39748
Total number of polymer chains4
Total molecular weight50141.92
Tsutakawa, S.E.,Classen, S.,Chapados, B.R.,Arvai, A.,Finger, D.L.,Guenther, G.,Tomlinson, C.G.,Thompson, P.,Sarker, A.H.,Shen, B.,Cooper, P.K.,Grasby, J.A.,Tainer, J.A. (deposition date: 2011-01-06, release date: 2011-04-27, Last modification date: 2011-07-13)
Primary citation
Tsutakawa, S.E.,Classen, S.,Chapados, B.R.,Arvai, A.S.,Finger, L.D.,Guenther, G.,Tomlinson, C.G.,Thompson, P.,Sarker, A.H.,Shen, B.,Cooper, P.K.,Grasby, J.A.,Tainer, J.A.
Human Flap Endonuclease Structures, DNA Double-Base Flipping, and a Unified Understanding of the FEN1 Superfamily.
Cell(Cambridge,Mass.), 145:198-211, 2011
PubMed: 21496641 (PDB entries with the same primary citation)
DOI: 10.1016/j.cell.2011.03.004
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.204100.6%0.7%6.9%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
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