3Q8K

Crystal Structure of Human Flap Endonuclease FEN1 (WT) in complex with product 5'-flap DNA, SM3+, and K+

Summary for 3Q8K

Related3Q8L 3Q8M
DescriptorFlap endonuclease 1, DNA (5'-D(*AP*CP*TP*CP*TP*GP*CP*CP*TP*CP*AP*AP*GP*AP*CP*GP*GP*T)-3'), DNA (5'-D(P*TP*GP*AP*GP*GP*CP*AP*GP*AP*GP*T)-3'), ... (8 entities in total)
Functional Keywordshelix-3 turn-helix, hydrophobic wedge, 3' flap binding site, hydrolase-dna complex, dna repair, replication, flap endonuclease, fen, fen1, dna, nuclease, 5' flap, ss-dsdna junction, helix-2 turn-helix, h2th, h3th, divalent cation, helical gateway, cap, acid block, two metal mechanism, unpaired, 5' nuclease, human, long patch base excision repair, hydrolase/dna
Biological sourceHomo sapiens (human)
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Cellular locationNucleus, nucleolus P39748
Total number of polymer chains4
Total molecular weight50141.92
Authors
Tsutakawa, S.E.,Classen, S.,Chapados, B.R.,Arvai, A.,Finger, D.L.,Guenther, G.,Tomlinson, C.G.,Thompson, P.,Sarker, A.H.,Shen, B.,Cooper, P.K.,Grasby, J.A.,Tainer, J.A. (deposition date: 2011-01-06, release date: 2011-04-27, Last modification date: 2011-07-13)
Primary citation
Tsutakawa, S.E.,Classen, S.,Chapados, B.R.,Arvai, A.S.,Finger, L.D.,Guenther, G.,Tomlinson, C.G.,Thompson, P.,Sarker, A.H.,Shen, B.,Cooper, P.K.,Grasby, J.A.,Tainer, J.A.
Human Flap Endonuclease Structures, DNA Double-Base Flipping, and a Unified Understanding of the FEN1 Superfamily.
Cell(Cambridge,Mass.), 145:198-211, 2011
PubMed: 21496641 (PDB entries with the same primary citation)
DOI: 10.1016/j.cell.2011.03.004
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2.2001 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.204100.6%0.7%6.9%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
Download full validation reportDownload