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3Q8K

Crystal Structure of Human Flap Endonuclease FEN1 (WT) in complex with product 5'-flap DNA, SM3+, and K+

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSSRL BEAMLINE BL11-1
Synchrotron siteSSRL
BeamlineBL11-1
Temperature [K]90
Detector technologyCCD
Collection date2010-04-01
DetectorMARMOSAIC 325 mm CCD
Wavelength(s)0.9796
Spacegroup nameP 31 2 1
Unit cell lengths105.438, 105.438, 105.064
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution34.460 - 2.200
R-factor0.1702
Rwork0.168
R-free0.21260
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1rxw
RMSD bond length0.012
RMSD bond angle0.864
Data reduction softwareMOSFLM
Data scaling softwareSCALA (3.2.25)
Phasing softwarePHASER
Refinement softwarePHENIX (1.7_629)
Data quality characteristics
 OverallInner shellOuter shell
Low resolution limit [Å]34.46234.5032.320
High resolution limit [Å]2.2006.9602.200
Rmerge0.0830.0490.557
Number of reflections34291
<I/σ(I)>7.5130.6
Completeness [%]99.179.5100
Redundancy3.63.43.6
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION728816% mPEG 2k, 20% saturated KCl, 0.5 mM Sm2(SO4)3, 10 mM Mg(NO3)2, 100 mM Hepes pH 7.0, 5% ethylene glycol, VAPOR DIFFUSION, temperature 288K
1VAPOR DIFFUSION728816% mPEG 2k, 20% saturated KCl, 0.5 mM Sm2(SO4)3, 10 mM Mg(NO3)2, 100 mM Hepes pH 7.0, 5% ethylene glycol, VAPOR DIFFUSION, temperature 288K

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