3Q4F

Crystal structure of xrcc4/xlf-cernunnos complex

Summary for 3Q4F

• Entry DOI: 10.2210/pdb3q4f/pdb
• Descriptor: Non-homologous end-joining factor 1, DNA repair protein XRCC4 (2 entities in total)
• Functional Keywords: dsb repair, nuclear, recombination-recombination complex, dna binding protein-protein binding complex, dna binding protein/protein binding
• Biological source: Homo sapiens (human); More
• Cellular location: Nucleus: Q9H9Q4 Q13426
• Total number of polymer chains: 8
• Total formula weight: 191518.09

Authors

Ropars, V.,Legrand, P.,Charbonnier, J.B. (deposition date: 2010-12-23, release date: 2011-08-03, Last modification date: 2024-02-21)

Primary citation

Ropars, V.,Drevet, P.,Legrand, P.,Baconnais, S.,Amram, J.,Faure, G.,Marquez, J.A.,Pietrement, O.,Guerois, R.,Callebaut, I.,Le Cam, E.,Revy, P.,de Villartay, J.P.,Charbonnier, J.B.
Structural characterization of filaments formed by human Xrcc4-Cernunnos/XLF complex involved in nonhomologous DNA end-joining.
Proc.Natl.Acad.Sci.USA, 108:12663-12668, 2011

PubMed Abstract: Cernunnos/XLF is a core protein of the nonhomologous DNA end-joining (NHEJ) pathway that processes the majority of DNA double-strand breaks in mammals. Cernunnos stimulates the final ligation step catalyzed by the complex between DNA ligase IV and Xrcc4 (X4). Here we present the crystal structure of the X4(1-157)-Cernunnos(1-224) complex at 5.5-Å resolution and identify the relative positions of the two factors and their binding sites. The X-ray structure reveals a filament arrangement for X4(1-157) and Cernunnos(1-224) homodimers mediated by repeated interactions through their N-terminal head domains. A filament arrangement of the X4-Cernunnos complex was confirmed by transmission electron microscopy analyses both with truncated and full-length proteins. We further modeled the interface and used structure-based site-directed mutagenesis and calorimetry to characterize the roles of various residues at the X4-Cernunnos interface. We identified four X4 residues (Glu(55), Asp(58), Met(61), and Phe(106)) essential for the interaction with Cernunnos. These findings provide new insights into the molecular bases for stimulatory and bridging roles of Cernunnos in the final DNA ligation step.

PubMed: 21768349
DOI: 10.1073/pnas.1100758108

Experimental method

X-RAY DIFFRACTION (5.5 Å)