Engineering Domain-Swapped Binding Interfaces by Mutually Exclusive Folding: Insertion of Ubiquitin into position 103 of Barnase

Summary for 3Q3F

DescriptorRibonuclease/Ubiquitin chimeric protein, SULFATE ION (3 entities in total)
Functional Keywordsdomain swap, oligomerization, ubiquitin insertion, hydrolase, protein binding
Biological sourceBacillus amyloliquefaciens
Cellular locationSecreted P00648
Total number of polymer chains1
Total molecular weight22665.72
Ha, J.-H.,Karchin, J.M.,Walker-Kopp, N.,Huang, L.-S.,Berry, E.A.,Loh, S.N. (deposition date: 2010-12-21, release date: 2012-01-25, Last modification date: 2017-11-08)
Primary citation
Ha, J.H.,Karchin, J.M.,Walker-Kopp, N.,Huang, L.S.,Berry, E.A.,Loh, S.N.
Engineering domain-swapped binding interfaces by mutually exclusive folding.
J.Mol.Biol., 416:495-502, 2012
PubMed: 22245575 (PDB entries with the same primary citation)
DOI: 10.1016/j.jmb.2011.12.050
MImport into Mendeley
Experimental method
NMR Information

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.228402.5%0.5%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
Download full validation report