3Q3F

Engineering Domain-Swapped Binding Interfaces by Mutually Exclusive Folding: Insertion of Ubiquitin into position 103 of Barnase

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003723	molecular_function	RNA binding
A	0004521	molecular_function	RNA endonuclease activity
A	0004540	molecular_function	RNA nuclease activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	9
Details	BINDING SITE FOR RESIDUE SO4 A 189

Chain	Residue
A	ALA1
A	GLN2
A	VAL3
A	ARG146
A	ARG176
A	LEU177
A	ARG178
A	HOH235
A	HOH298

---

site_id	AC2
Number of Residues	9
Details	BINDING SITE FOR RESIDUE SO4 A 190

Chain	Residue
A	LYS62
A	ASN77
A	THR79
A	GLY181
A	HOH276
A	HOH291
A	HOH326
A	HOH365
A	HOH420

---

site_id	AC3
Number of Residues	7
Details	BINDING SITE FOR RESIDUE SO4 A 191

Chain	Residue
A	ARG146
A	GLN153
A	ARG176
A	HOH256
A	HOH313
A	HOH395
A	HOH402

---

site_id	AC4
Number of Residues	8
Details	BINDING SITE FOR RESIDUE SO4 A 192

Chain	Residue
A	SER57
A	ARG59
A	THR118
A	LEU119
A	GLU120
A	LYS137
A	HOH308
A	HOH429

---

site_id	AC5
Number of Residues	10
Details	BINDING SITE FOR RESIDUE SO4 A 193

Chain	Residue
A	LYS27
A	GLU73
A	ARG83
A	ARG87
A	HIS102
A	GLY105
A	GLU120
A	HOH397
A	HOH424
A	HOH450

---

site_id	AC6
Number of Residues	11
Details	BINDING SITE FOR RESIDUE SO4 A 194

Chain	Residue
A	TRP35
A	ALA37
A	SER38
A	LYS39
A	GLY40
A	PHE82
A	ARG83
A	LYS167
A	HOH250
A	HOH268
A	HOH335

---

site_id	AC7
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 195

Chain	Residue
A	ARG72
A	SER92
A	HOH356
A	HOH421

---

site_id	AC8
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 A 196

Chain	Residue
A	ARG72
A	HOH387

---

site_id	AC9
Number of Residues	8
Details	BINDING SITE FOR RESIDUE SO4 A 197

Chain	Residue
A	THR6
A	PHE7
A	ASP8
A	ASN77
A	HOH378
A	HOH415
A	HOH422
A	HOH425

---

site_id	BC1
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 198

Chain	Residue
A	HIS18
A	TRP94
A	ALA150
A	HOH227

---

site_id	BC2
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 A 199

Chain	Residue
A	ARG59
A	HOH249
A	HOH380

---

site_id	BC3
Number of Residues	3
Details	BINDING SITE FOR RESIDUE SO4 A 200

Chain	Residue
A	THR113
A	LYS115
A	HOH389

---

site_id	BC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 A 201

Chain	Residue
A	GLU155
A	ARG158
A	HOH245
A	HOH325

---

site_id	BC5
Number of Residues	2
Details	BINDING SITE FOR RESIDUE SO4 A 202

Chain	Residue
A	LYS152
A	GLN153

---

site_id	BC6
Number of Residues	6
Details	BINDING SITE FOR RESIDUE SO4 A 203

Chain	Residue
A	PHE149
A	SER169
A	THR170
A	HOH255
A	HOH261
A	HOH340

---

site_id	BC7
Number of Residues	5
Details	BINDING SITE FOR RESIDUE SO4 A 204

Chain	Residue
A	GLY65
A	ARG69
A	HOH210
A	HOH229
A	HOH231

---

Functional Information from PROSITE/UniProt

site_id	PS00299
Number of Residues	26
Details	UBIQUITIN_1 Ubiquitin domain signature. KakIqDkegIPpdqQrLIFaGkqleD

Chain	Residue	Details
A	LYS131-ASP156

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	Active site: {"description":"Proton acceptor"}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	Active site: {"description":"Proton donor"}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	75
Details	Domain: {"description":"Ubiquitin-like 8","evidences":[{"source":"PROSITE-ProRule","id":"PRU00214","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---