3Q3F

Engineering Domain-Swapped Binding Interfaces by Mutually Exclusive Folding: Insertion of Ubiquitin into position 103 of Barnase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Ribonuclease/Ubiquitin chimeric protein	polymer	189	21128.7	1	UniProt (P00648) UniProt (P0CG48) Pfam (PF00545) Pfam (PF00240)	Bacillus amyloliquefaciens
2	B, C, D, E, F... (A)	SULFATE ION	non-polymer		96.1	16	Chemie (SO4)
3	R (A)	water	water		18.0	260	Chemie (HOH)

Sequence modifications

A: 1 - 103 (UniProt: P00648)

PDB	External Database	Details
Met 0	-	initiating methionine

A: 106 - 180 (UniProt: P0CG48)

PDB	External Database	Details
Gly 104	-	linker
Gly 105	-	linker

A: 182 - 188 (UniProt: P00648)

PDB	External Database	Details
Gly 181	-	linker

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	21128.7
Non-Polymers*	Number of molecules	16
	Total formula weight	1537.0
All*	Total formula weight	22665.7

*Water molecules are not included.