3Q2S

Crystal Structure of CFIm68 RRM/CFIm25 complex

3Q2S の概要

• エントリーDOI: 10.2210/pdb3q2s/pdb
• 関連するPDBエントリー: 3Q2T
• 分子名称: Cleavage and polyadenylation specificity factor subunit 5, Cleavage and polyadenylation specificity factor subunit 6 (3 entities in total)
• 機能のキーワード: cfim, cfim25, cfim68, cpsf5, cpsf6, cpsf, 3' end processing, rna processing, cleavage factor, nudix protein, protein-protein complex, rrm domain, nudix fold, rna, nuclear protein
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Nucleus : O43809 Q16630
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 97033.13

構造登録者

Yang, Q.,Coseno, M.,Gilmartin, G.M.,Doublie, S. (登録日: 2010-12-20, 公開日: 2011-02-16, 最終更新日: 2023-09-13)

主引用文献

Yang, Q.,Coseno, M.,Gilmartin, G.M.,Doublie, S.
Crystal Structure of a Human Cleavage Factor CFI(m)25/CFI(m)68/RNA Complex Provides an Insight into Poly(A) Site Recognition and RNA Looping.
Structure, 19:368-377, 2011

PubMed Abstract: Cleavage factor I(m) (CFI(m)) is a highly conserved component of the eukaryotic mRNA 3' processing machinery that functions in sequence-specific poly(A) site recognition through the collaboration of a 25 kDa subunit containing a Nudix domain and a larger subunit of 59, 68, or 72 kDa containing an RNA recognition motif (RRM). Our previous work demonstrated that CFI(m)25 is both necessary and sufficient for sequence-specific binding of the poly(A) site upstream element UGUA. Here, we report the crystal structure of CFI(m)25 complexed with the RRM domain of CFI(m)68 and RNA. The CFI(m)25 dimer is clasped on opposite sides by two CFI(m)68 RRM domains. Each CFI(m)25 subunit binds one UGUA element specifically. Biochemical analysis indicates that the CFI(m)68 RRMs serve to enhance RNA binding and facilitate RNA looping. The intrinsic ability of CFI(m) to direct RNA looping may provide a mechanism for its function in the regulation of alternative poly(A) site selection.

PubMed: 21295486
DOI: 10.1016/j.str.2010.12.021

実験手法

X-RAY DIFFRACTION (2.9 Å)