3Q2S

Crystal Structure of CFIm68 RRM/CFIm25 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2010-02-23
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 21 3
Unit cell lengths	139.323, 139.323, 139.323
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.903 - 2.900
R-factor	0.2153
Rwork	0.213
R-free	0.27760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3bho
RMSD bond length	0.005
RMSD bond angle	0.943
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX (1.6_289)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.092
Number of reflections	20194
<I/σ(I)>	10.7
Completeness [%]	100.0	100
Redundancy	22.5	16.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	20% PEG 3350, 0.2M Magnesium Formate, 0.05M HEPES pH 7.0, vapor diffusion, hanging drop, temperature 298K