3PWK

Crystal Structure of Aspartate beta-Semialdehide Dehydrogenase from Streptococcus pneumoniae with 2',5'-Adenosine diphosphate and D-2-aminoadipate

3PWK の概要

• エントリーDOI: 10.2210/pdb3pwk/pdb
• 関連するPDBエントリー: 2GZ2 3PWS 3PYL 3PYX 3PZB 3PZR 3Q0E 3Q11 3Q1L
• 分子名称: Aspartate-semialdehyde dehydrogenase, 5'-O-MONOPHOSPHORYLADENYLYL(2'->5')ADENYLYL(2'->5')ADENOSINE, trans-cyclohexane-1,4-dicarboxylic acid, ... (6 entities in total)
• 機能のキーワード: dehydrogenase, nadp binding, oxidoreductase-oxidoreductase inhibitor complex, oxidoreductase/oxidoreductase inhibitor
• 由来する生物種: Streptococcus pneumoniae
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 82975.19

構造登録者

Pavlovsky, A.G.,Viola, R.E. (登録日: 2010-12-08, 公開日: 2012-01-04, 最終更新日: 2023-09-13)

主引用文献

Pavlovsky, A.G.,Liu, X.,Faehnle, C.R.,Potente, N.,Viola, R.E.
Structural Characterization of Inhibitors with Selectivity against Members of a Homologous Enzyme Family.
Chem.Biol.Drug Des., 79:128-136, 2012

PubMed Abstract: The aspartate biosynthetic pathway provides essential metabolites for many important biological functions, including the production of four essential amino acids. As this critical pathway is only present in plants and microbes, any disruptions will be fatal to these organisms. An early pathway enzyme, l-aspartate-β-semialdehyde dehydrogenase, produces a key intermediate at the first branch point of this pathway. Developing potent and selective inhibitors against several orthologs in the l-aspartate-β-semialdehyde dehydrogenase family can serve as lead compounds for antibiotic development. Kinetic studies of two small molecule fragment libraries have identified inhibitors that show good selectivity against l-aspartate-β-semialdehyde dehydrogenases from two different bacterial species, Streptococcus pneumoniae and Vibrio cholerae, despite the presence of an identical constellation of active site amino acids in this homologous enzyme family. Structural characterization of enzyme-inhibitor complexes have elucidated different modes of binding between these structurally related enzymes. This information provides the basis for a structure-guided approach to the development of more potent and more selective inhibitors.

PubMed: 22039970
DOI: 10.1111/j.1747-0285.2011.01267.x

実験手法

X-RAY DIFFRACTION (1.5 Å)