3PWS

Crystal Structure of Aspartate beta-Semialdehide Dehydrogenase from Streptococcus pneumoniae with 2',5'-Adenosine diphosphate and D-2-aminoadipate

Summary for 3PWS

• Entry DOI: 10.2210/pdb3pws/pdb
• Related: 2GZ2 3PWK 3PYL 3PYX 3PZB 3PZR 3Q0E 3Q11 3Q1L
• Descriptor: Aspartate-semialdehyde dehydrogenase, (2R)-2-aminohexanedioic acid, SODIUM ION, ... (5 entities in total)
• Functional Keywords: dehydrogenase, nadp, oxidoreductase-oxidoreductase inhibitor complex, oxidoreductase/oxidoreductase inhibitor
• Biological source: Streptococcus pneumoniae
• Total number of polymer chains: 2
• Total formula weight: 81276.75

Authors

Pavlovsky, A.G.,Viola, R.E. (deposition date: 2010-12-08, release date: 2012-01-04, Last modification date: 2024-02-21)

Primary citation

Pavlovsky, A.G.,Liu, X.,Faehnle, C.R.,Potente, N.,Viola, R.E.
Structural Characterization of Inhibitors with Selectivity against Members of a Homologous Enzyme Family.
Chem.Biol.Drug Des., 79:128-136, 2012

PubMed Abstract: The aspartate biosynthetic pathway provides essential metabolites for many important biological functions, including the production of four essential amino acids. As this critical pathway is only present in plants and microbes, any disruptions will be fatal to these organisms. An early pathway enzyme, l-aspartate-β-semialdehyde dehydrogenase, produces a key intermediate at the first branch point of this pathway. Developing potent and selective inhibitors against several orthologs in the l-aspartate-β-semialdehyde dehydrogenase family can serve as lead compounds for antibiotic development. Kinetic studies of two small molecule fragment libraries have identified inhibitors that show good selectivity against l-aspartate-β-semialdehyde dehydrogenases from two different bacterial species, Streptococcus pneumoniae and Vibrio cholerae, despite the presence of an identical constellation of active site amino acids in this homologous enzyme family. Structural characterization of enzyme-inhibitor complexes have elucidated different modes of binding between these structurally related enzymes. This information provides the basis for a structure-guided approach to the development of more potent and more selective inhibitors.

PubMed: 22039970
DOI: 10.1111/j.1747-0285.2011.01267.x

Experimental method

X-RAY DIFFRACTION (2 Å)