3P7O

Rat Insulin Degrading Enzyme (Insulysin) E111F mutant with two bound peptides

Summary for 3P7O

Entry DOI	10.2210/pdb3p7o/pdb
Descriptor	Insulin-degrading enzyme, active site bound peptide, distal site bound peptide, ... (4 entities in total)
Functional Keywords	metallopeptidase, hydrolase
Biological source	Rattus norvegicus (brown rat,rat,rats) More
Cellular location	Cytoplasm: P35559
Total number of polymer chains	3
Total formula weight	119195.49
Authors	Rodgers, D.W.,Noinaj, N. (deposition date: 2010-10-12, release date: 2011-07-27, Last modification date: 2024-02-21)
Primary citation	Noinaj, N.,Bhasin, S.K.,Song, E.S.,Scoggin, K.E.,Juliano, M.A.,Juliano, L.,Hersh, L.B.,Rodgers, D.W. Identification of the allosteric regulatory site of insulysin. Plos One, 6:e20864-e20864, 2011 Cited by PubMed Abstract: Insulin degrading enzyme (IDE) is responsible for the metabolism of insulin and plays a role in clearance of the Aβ peptide associated with Alzheimer's disease. Unlike most proteolytic enzymes, IDE, which consists of four structurally related domains and exists primarily as a dimer, exhibits allosteric kinetics, being activated by both small substrate peptides and polyphosphates such as ATP. PubMed: 21731629 DOI: 10.1371/journal.pone.0020864 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.1423 Å)

Structure validation